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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Pre-hydrolysis improves utilisation of dietary protein in the larval teleost Atlantic halibut (Hippoglossus hippoglossus L.)
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Pre-hydrolysis improves utilisation of dietary protein in the larval teleost Atlantic halibut (Hippoglossus hippoglossus L.)

机译：预水解可提高幼虫硬骨大西洋大比目鱼（Hippoglossus hippoglossus L.）中膳食蛋白质的利用率

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摘要

A protein preparation labelled by incorporation of [U]~(14)C-AA was hydrolysed to various degrees and administered to a teleost fish larva (Atlantic halibut, Hippoglossus hippoglossus L.) by tube-feeding, and its post-administration utilisation was studied. Three treatments were prepared: IntP—intact protein, PHP—pepsin-hydrolysed protein, and HHP—highly hydrolysed protein (using pepsin, trypsin, endoproteinase Glu-C, Asp-N, and Pro-C). At small doses (11.4+1.5 μg larvae~(-1)), the intact protein (IntP) was digested and absorbed to 36+5.5%. However, the relative absorption efficiency of the intact protein was reduced as the dose increased. Absorption efficiency was higher when the protein was hydrolysed prior to feeding the larvae and was constant at 63% (R~2=98) independent of degree of proteolysis and dose (ranging from 3.5 to 35 μg larvae~(-1)). The initial absorption rate increased with the degree of hydrolysis. Calculations based on data collected during the first 30-120 min show that the absorption of PHP and HHP into extra-intestinal body tissues was 2.2 and 3 times as fast, respectively, as that of intact protein. However, the rates of absorption did not influence the distribution of absorbed AA into either catabolism or anabolism, as all larvae, independent of which protein solution they were given, catabolised 42±7% of the absorbed AA, and accumulated 49±6% into the body tissue, during the 20 h post-feeding incubation period. Larval age and size did not influence the absorption and utilisation of the hydrolysed protein preparations. This was different from the intact protein, as significantly higher fractions of the intact protein were absorbed by the larvae at 31 days past first feeding (dpff) than by larvae at 25 dpff. Analysis of the faecal evacuation suggested that the poor protein utilisation in the younger larvae was due to enhanced faecal evacuation, which in some larvae was more than 50% only 4 h post diet administration, at a time when the process of digestion and absorption was far from complete. This indicated that faecal evacuation is a critical factor in the utilisation of slowly digested and absorbed feed components, such as intact proteins, by fish larvae.
机译：将通过掺入[U]〜（14）C-AA标记的蛋白质制剂进行不同程度的水解，然后通过管饲法将其施用至硬骨鱼幼体（大西洋大比目鱼，Hippoglossus hippoglossus L.）中，给药后的利用率为研究。准备了三种处理方法：IntP（完整蛋白），PHP（胃蛋白酶水解蛋白）和HHP（高度水解蛋白）（使用胃蛋白酶，胰蛋白酶，内切蛋白酶Glu-C，Asp-N和Pro-C）。小剂量（11.4 + 1.5μg幼虫〜（-1））时，完整蛋白（IntP）被消化并吸收至36 + 5.5％。但是，随着剂量的增加，完整蛋白的相对吸收效率会降低。蛋白质在喂食幼虫之前先水解，吸收效率更高，并且恒定在63％（R〜2 = 98），与蛋白水解程度和剂量无关（从3.5至35μg幼虫〜（-1））。初始吸收率随水解度的增加而增加。根据在最初30-120分钟内收集的数据进行的计算表明，PHP和HHP在肠道外人体组织中的吸收速度分别是完整蛋白质的2.2倍和3倍。然而，吸收速率并没有影响吸收的AA分解代谢或合成代谢的分布，因为所有幼虫，不管给予哪种蛋白质溶液，都分解代谢了42±7％的AA，并累积了49±6％。喂食后20小时的温育期间，身体组织。幼虫的年龄和大小不影响水解蛋白制品的吸收和利用。这与完整蛋白不同，因为幼虫在第一次进食（dpff）后31天的吸收量要比25 dpff时的幼虫高得多。粪便排泄的分析表明，幼虫幼虫蛋白质利用不良是由于粪便排泄增加，在某些幼虫中，仅在饮食管理4小时后，即消化和吸收过程很远的时候，粪便排泄却超过50％。从完成。这表明排泄粪便是鱼幼虫利用缓慢消化和吸收的饲料成分（例如完整蛋白）的关键因素。

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