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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Thermal history-dependent expression of the hsp70 gene in purple sea urchins: Biogeographic patterns and the effect of temperature acclimation
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Thermal history-dependent expression of the hsp70 gene in purple sea urchins: Biogeographic patterns and the effect of temperature acclimation

机译：热史依赖的hsp70基因在紫海胆中的表达：生物地理模式和温度适应的影响

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摘要

Thermal variation associated with large spatial scales is thought to significantly affect the physiology of organisms in marine systems and consequently limit their distribution. To examine this phenomenon, this study compared the heat shock protein (hsp70) mRNA expression of purple sea urchins (Strongylocentrotus purpuratus) collected from different geographic locations and those acclimated to different temperatures. Tube feet were removed from S. purpuratus freshly collected from Fogarry Creek, Oregon (FC-OR) and Carpinteria, California (CRP-CA) and from urchins acclimated to 5℃ and 20℃ in the laboratory. Tissue samples were then immediately frozen or incubated at various temperatures between 5℃ and 36℃ for 30 min in vitro. Real-time PCR was used to measure the hsp70 mRNA levels of each sample. The results showed evidence for differential regulation of hsp70 between S. purpuratus in both field and laboratory studies. For example, the incubation temperature that induced maximum hsp70 mRNA expression (T_(max)) was higher in CRP-CA urchins (26℃) than those from FC-OR (23℃). Additionally, although the T_(max) of the two acclimation groups was identical (26℃), S. purpuratus acclimated to 5℃ failed to induce hsp70 mRNA after incubation at 32℃, while tube feet of the 20℃-acclimated urchins remained transcriptionally active. In general, the extent of hsp70 mRNA induction was substantial, exceeding 250-fold in the 5℃-acclimated urchin tissues after incubation at 26℃. These data suggest that the thermal variation encountered across S. purpuratus' range is sufficient to effect the regulation of thermally sensitive genes, like hsp70.
机译：人们认为，与大空间尺度相关的热变化会严重影响海洋系统中生物的生理，因此限制了它们的分布。为了检验这种现象，本研究比较了从不同地理位置和适应不同温度的紫海胆（Strongylocentrotus purpuratus）的热激蛋白（hsp70）mRNA表达。从刚从俄勒冈州的Fogarry Creek（FC-OR）和加利福尼亚的Carpinteria（CRP-CA）以及实验室中适应于5℃和20℃的海胆中新鲜采集的紫葡萄链球菌中取出管脚。然后将组织样品立即冷冻或在5℃至36℃的不同温度下体外培养30分钟。实时PCR用于测量每个样品的hsp70 mRNA水平。结果表明，在野外和实验室研究中，紫癜链球菌对hsp70的差异调节都有证据。例如，在CRP-CA顽童（26℃）中诱导hsp70 mRNA最大表达（T_（max））的温育温度比在FC-OR（23℃）中高。另外，尽管两个适应组的T_（max）相同（26℃），但适应于5℃的紫癜链霉菌在32℃温育后未能诱导hsp70 mRNA，而适应20℃的海胆的管脚仍转录活性。通常，在26℃温育后，在5℃适应的野孩子组织中，hsp70 mRNA的诱导程度是相当大的，超过250倍。这些数据表明，在紫癜链球菌的整个范围内遇到的热变化足以影响热敏感基因（如hsp70）的调控。

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