• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Toxicity comparison among four strains of Margalefidinium polykrikoides from China, Malaysia, and USA (belonging to two ribotypes) and possible implications
【24h】

Toxicity comparison among four strains of Margalefidinium polykrikoides from China, Malaysia, and USA (belonging to two ribotypes) and possible implications

机译：中国，马来西亚和美国的四种Margalefidinium polykrikoides菌株（属于两种核糖型）的毒性比较及其可能的意义

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The unarmored dinoflagellate Margalefidinium polykrikoides is a well-known cosmopolitan harmful alga that forms intensive blooms causing massive fish kills and mortality of other marine animals. Globally distributed populations of M. polykrikoides have been categorized into four ribotypes: East Asian, American/Malaysian, Philippines, and Mediterranean, according to their characteristic sequences in LSU rRNA gene and primary geographic distributions, while no discernible morphological feature has been observed to distinguish. Here we examined the toxicity of four strains of M. polykrikoides including MPJZB-C3 and MPJZB-D6 (East Asian ribotype, from China), CP1 (American/Malaysian ribotype, from USA), and MPCoKK23 (American/Malaysian ribotype, from Malaysia), to three aquatic animals including the marine medaka (Oryzias melastigma), rotifer (Brachionus plicatilis, L-type), and brine shrimp (Artemia salina). With equivalent initial cell densities ranged from 1000 to 7000 cells mL(-1), CP1 and MPCoKK23 displayed acute toxicity in a dose-response manner to the three target animals (100% mortality at higher cell densities) within 24 h, MPJZB-C3 also displayed acute toxicity to the fish and rotifer in 24 h (up to 50% mortality) when the cell densities were above 3000 cells mL(-1). The strain MPJZB-D6 did not exhibit significant toxicity to all three animals in 24 h, but, in 120 h, caused 50-100% mortalities in fish when the cell density was > 3000 cells mL(-1) and 17% mortality in brine shrimp when the cell density was > 5000 cells mL(-1). Statistical analysis of the data indicated that the strains CP1 and MPCoKK23 exhibited significantly higher toxicity (50-100% mortalities) to all three animals than the strains MPJZB-C3 and MPJZB-D6 (0-100% mortality) did within 24 h exposure, while, however, the difference in toxicity between the American and Malaysian strains and that between the two Chinese strains were both not significant in terms of the mortality of test animals. While the cell-free culture media (by filtration) and sonicated cultures were less toxic to test animals than the live cultures for all M. polykrikoides strains, sonicated cultures were even less toxic than the cell-free culture media. Among the three target animals, the marine medaka exhibited most sensitive to the toxicity, followed by rotifer and then brine shrimp. Collectively, while our results demonstrated that all strains, or both ribotypes, of M. polykrikoides are toxic, the results may also suggest the differences in toxicity to three test animals among the four cultures of M. polykrikoides reflect the inherent difference in toxicity between East Asian and American/Malaysian ribotypes, which stimulated us to propose a further investigation on whether or not the four different ribotypes also differ inherently in their toxicity in the future, via comparing more strains of all four ribotypes and relevant genetic characterizations.
机译：未装甲的鞭毛藻Margalefidinium polykrikoides是一种众所周知的世界性有害藻类，会形成密集的水华，导致大量的鱼类死亡和其他海洋动物死亡。根据LSU rRNA基因的特征序列和主要地理分布，全球分布的多核克雷伯氏菌种群已分为四种核糖型：东亚，美洲/马来西亚，菲律宾和地中海，而未观察到可辨别的形态学特征。在这里，我们研究了四株多核分枝杆菌的毒性，包括MPJZB-C3和MPJZB-D6（来自中国的东亚核糖体），CP1（来自美国的美国/马来西亚核糖体）和MPCoKK23（来自马来西亚的美国/马来西亚核糖体）的毒性。 ），三种水生动物，包括海洋the（Oryzias melastigma），轮虫（Brachionus plicatilis，L型）和卤水虾（卤虫）。当CP1和MPCoKK23的等效初始细胞密度范围为1000至7000个细胞mL（-1）时，在24小时内对三只靶动物（较高细胞密度下的死亡率为100％）以剂量-反应方式显示出急性毒性，MPJZB-C3当细胞密度超过3000细胞mL（-1）时，在24小时内对鱼类和轮虫也显示出急性毒性（高达50％的死亡率）。 MPJZB-D6菌株在24小时内未对所有三只动物均表现出明显的毒性，但在120小时内，当细胞密度> 3000细胞mL（-1）时，鱼死亡的比例为50-100％，而在30h时死亡率为17％细胞密度> 5000个细胞mL（-1）时用盐水虾。数据的统计分析表明，相比于MPJZB-C3和MPJZB-D6菌株（0-100％死亡率），CP1和MPCoKK23菌株对所有三只动物均显示出更高的毒性（50-100％死亡率），然而，就测试动物的死亡率而言，美国和马来西亚品系之间以及中国的两种品系之间的毒性差异均不显着。尽管无细胞培养基（通过过滤）和超声处理的培养物对测试动物的毒性小于所有多核分支杆菌菌株的活培养物，但超声处理的毒性甚至比无细胞培养基低。在这三种目标动物中，海洋中高中对毒性最敏感，其次是轮虫，然后是盐水虾。总的来说，尽管我们的结果表明多核克雷伯氏菌的所有菌株或两种核糖型都是有毒的，但结果也可能表明四种多核克雷伯氏菌对三种试验动物的毒性差异反映了东部亚洲和美国/马来西亚的核糖型激发了我们提出一种进一步的研究，即通过比较所有四种核糖型的更多菌株和相关的遗传学特征，进一步研究四种不同的核糖型在未来是否也固有地具有不同的毒性。

著录项

  • 来源
    《Journal of Experimental Marine Biology and Ecology》 |2020年第3期|151293.1-151293.8|共8页
  • 作者

  • 作者单位

    Chinese Acad Sci Inst Oceanol CAS Key Lab Marine Ecol & Environm Sci Qingdao 266071 Peoples R China|Univ Chinese Acad Sci Beijing 100049 Peoples R China;

    Chinese Acad Sci Inst Oceanol CAS Key Lab Marine Ecol & Environm Sci Qingdao 266071 Peoples R China|Qingdao Natl Lab Marine Sci & Technol Lab Marine Ecol & Environm Sci Qingdao 266237 Peoples R China|Chinese Acad Sci Ctr Ocean Megasci Qingdao 266071 Peoples R China;

    Univ Malaya Inst Ocean & Earth Sci Bachok Marine Res Stn Bachok 16310 Kelantan Malaysia;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Dinoflagellate; Harmful algal blooms (HABs); Margalefidinium polykrikoides; Ribotype; Toxicity;

    机译：鞭毛藻;有害藻华（HABs）;Margalefidinium polykrikoides;核型毒性;

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号