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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Individual growth rate (IGR) and aminoacyl-tRNA synthetases (AARS) activity as individual-based indicators of growth rate of North Pacific krill, Euphausia pacifica
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Individual growth rate (IGR) and aminoacyl-tRNA synthetases (AARS) activity as individual-based indicators of growth rate of North Pacific krill, Euphausia pacifica

机译：单个生长速率（IGR）和氨基酰基-TRNA合成酶（AARS）活性作为北太平洋克里尔的北太平洋克尔（Euphausia Pacifica）的个体增长速率指标

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摘要

We investigated aminoacyl-tRNA synthetases (AARS) activity and individual growth rate (IGR) as individual-based in situ indicators of growth in adult krill, Euphausia pacifica. AARS enzymes catalyze the first step in protein synthesis while the IGR method is based on changes in body length during molting. Growth rates of field-collected krill were measured via the IGR method and individuals were subsequently preserved for AARS analysis to yield paired measurements. Our results show that conditions during the IGR incubation period influenced AARS activity in these individuals precluding a direct comparison but revealing the different timescales across which these two measures integrate. Importantly, they show that AARS activity provides a snap-shot image of an organism's metabolism, while IGR of krill is thought to integrate their environmental experience over several days. Each method would require repeated measurements to estimate population growth rates integrated over seasonal or generational time scales. As part of this project, we investigated how specific the AARS assay is to protein synthesis by testing a modified protocol that includes an additional blank and found evidence that the current assay may be measuring other cellular processes in addition to its intended signal. Our results suggest that a new NADH Blank might be optimized to improve the specificity of the assay.
机译：我们研究了氨基酰基-TRNA合成酶（AARS）活性和单独的生长速率（IGr），以良好的成人KRILL，Euphausia Pacifica的增长指标。 AARS酶催化蛋白质合成的第一步，而IGR方法基于蜕皮期间的体长变化。通过IGR方法测量现场收集的KRILL的生长速率，随后将个体保留用于AARS分析以产生配对测量。我们的结果表明，IGR孵育期间的条件影响了这些个人中的AARS活动排除了直接比较，但揭示了这两项措施整合的不同时间尺寸。重要的是，他们表明，AARS活动提供了生物体的新陈代谢的快照图像，而克里尔的IGRITE被认为将它们的环境经验整合在几天内。每种方法都需要重复测量以估计整合季节性或世代时间尺度的人口增长率。作为该项目的一部分，我们研究了AARS测定如何通过测试包括额外坯料的修饰方案来对蛋白质合成，并发现除了其预期信号之外，当前测定可以测量当前测定可以测量其他细胞过程。我们的结果表明，可能优化新的NADH空白以改善测定的特殊性。

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