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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Diurnal variations in expression of photosynthesis-related proteins in the harmful Raphidophyceae Chattonella marina var. antiqua
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Diurnal variations in expression of photosynthesis-related proteins in the harmful Raphidophyceae Chattonella marina var. antiqua

机译：有害Raphidophycoe Chattonella Marina VAR中的光合作用相关蛋白表达的昼夜变异。 antiqua.

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Many biological features of Chattonella species display diurnal oscillations, but little is known about the corresponding cellular responses at the proteome level. In the present study, we hypothesized that the abundance of certain proteins would periodically fluctuate to suit the diurnal changes of biological processes in Chattonella cells. To WA this hypothesis, we undertook proteomic analysis over 7 time points covering a 24-h period in C. marina var. antiqua cells at early stationary phase (12:00, 17:00, 20:00, 24:00 on day 8, and 05:00, 08:00, 12:00 on the following day). The protein profiles of C. marina var. antiqua cells collected from the diurnal cycle shared a majority of common protein spots. However, the expression of 16 out of 124 detected spots exhibited significant differences between light and dark periods and/or over the 7 time points of the diurnal cycle. The proteins of OEE, Cyt c553, and AtpB showed significantly higher expression levels in the light period, and generally were maximally expressed during the period of 12:00-17:00. The proteins of GAPDH and RPL12 generally showed higher expression levels in the dark period, and were maximally expressed during the period of 20:00-24:00. These findings support our hypothesis, and suggest that the periodic expressions of these proteins may be critical either for optimal cell utilization of resources or to maintain proper cell function throughout diurnal cycles.
机译：多种突出物种的许多生物学特征显示昼夜振荡，但关于蛋白质组水平的相应细胞反应很少。在本研究中，我们假设某些蛋白质的丰度会如何定期波动，以适应Chattonella细胞中生物过程的昼夜变化。对于Wa这个假设，我们在C.Marina VAR中覆盖了覆盖了24小时的7个时间点进行蛋白质组学分析。 Antiqua细胞在早期固定阶段（12:00,17：00，20:00，第8天24:00，第二天第05:00，08:00，12:00）。 C. marina var的蛋白质谱。从昼夜循环收集的抗iqua细胞共享大部分常见的蛋白质斑点。然而，124个检测到的斑点中的16个表达在光和暗时期和/或在昼夜循环的7个时间点之间表现出显着差异。 OEE，Cyt C553和ATPB的蛋白质在光周期中显示出显着更高的表达水平，并且在12：00-17：00期间通常最大地表达。 GAPDH和RPL12的蛋白质通常在黑暗时期中显示出更高的表达水平，并且在20：00-24：00期间最大地表达。这些发现支持我们的假设，并表明这些蛋白质的周期性表达可能是用于最佳的细胞利用资源的关键或在整个昼夜循环中保持适当的细胞功能。

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