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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Sirtuin-dependent recovery from aerial heat shock: The effects of food ration, thermal history, and sirtuin inhibition on clearance rates and valve gape activity of the California mussel, Mytilus californianus (Conrad)
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Sirtuin-dependent recovery from aerial heat shock: The effects of food ration, thermal history, and sirtuin inhibition on clearance rates and valve gape activity of the California mussel, Mytilus californianus (Conrad)

机译：从空中休克的Sirtuin依赖恢复：食品口粮，热历史和SIRTUIN抑制对加州贻贝的清除率和瓣膜Gape活动的影响，Californianus（康拉德）

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Intertidal mussels regularly experience seasonal fluctuations in food availability and heat stress over the course of a tidal cycle, yet little is known about the biochemical mechanisms linking food ration and thermal tolerance, despite evidence that food enhances stress tolerance at the organismal level. We hypothesized that sirtuins (food-dependent protein regulators of cellular homeostasis), which may regulate the cellular stress response of mytilids during heat stress, also affect organismal performance during recovery from heat stress. As part of a broader study, we investigated the effects of acclimation food ration, acclimation emersion (air) temperature, and sirtuin inhibitors on the feeding behavior of the California mussel, Mytilus californianus, during recovery from acute aerial heat shock. Mussels were acclimated for 3 wk. to either a low (0.25% algae.g mussel dry wt(-1)) or high (1.5% algae.g mussel dry wt(-1)) algal ration and low (20 degrees C) or high (30 degrees C) emersion temperatures, in tidal simulator tanks capable of maintaining standard circadian and circatidal rhythms. Following acclimation, mussels were exposed to two sirtuin inhibitors (nicotinamide and suramin) or no inhibitors for one high tide cycle. During the subsequent low tide cycle, all mussels were exposed to an acute aerial heat shock (33 degrees C) and then allowed to recover in their respective acclimation conditions. Every 12 h during the 48 h before sirtuin inhibition and 48 h following acute heat shock (in the corresponding high tide periods), we recorded clearance rates and valve gape activity. Mussels acclimated to a low food ration significantly reduced clearance rates with heat shock and sirtuin inhibition, but not with heat shock alone, relative to pre-treatment values regardless of emersion temperature. Mussels acclimated to a low food ration and high emersion temperature showed significant reductions in valve gape activity following an acute heat shock following sirtuin inhibition, but not in any of the other acclimation groups. Unexpectedly, heat shock alone reduced clearance rates of mussels acclimated to high food and high emersion temperatures only, without any additional effect by heat shock and sirtuin inhibition. Together, these findings suggest an important role for sirtuins at the organismal level during recovery from heat shock following acclimation to a low food ration.
机译：跨境贻贝经常在潮汐循环过程中经常经历食品可用性和热压力的季节性波动，但是对于连接食品配量和热耐受性的生化机制，尽管有证据表明食物可以增强在有机体水平的胁迫耐受性的情况下，但令人讨厌。我们假设SIRTUIN（细胞静态的食物依赖性蛋白质调节剂），其可以调节热应激期间菌根细胞的细胞应激响应，也影响从热应激恢复过程中的有机体性能。作为更广泛的研究的一部分，我们研究了适应性食品口粮，适应性偏振（空气）温度和SIRTUIN抑制剂对加州贻贝，米蒂斯·加州植物的饲养行为的影响，从急性空中热休克中恢复。贻贝适应了3个wk。对于低（0.25％藻类贻贝干WT（-1））或高（1.5％藻类贻贝干WT（-1））藻类率和低（20℃）或高（30℃）潮汐温度，在潮汐模拟器罐中，能够维持标准昼夜节律和循环节奏。在适应时，贻贝暴露于两个SIRTUIN抑制剂（烟酰胺和苏里南）或没有抑制剂用于一个高潮循环。在随后的低潮循环期间，将所有贻贝暴露于急性天线热休克（33℃），然后在其各自的加速条件下恢复。每12小时在Sirtuin抑制前48小时和急性热冲击后48小时（在相应的高潮期间）后，我们记录了间隙率和阀门长化活动。贻贝适应低食物口粮，显着降低了热休克和SIRTUIN抑制的清除率，但不仅仅是单独的热冲击，而不管偏热温度如何。贻贝适应于低食物的较低，高源温度显示出在SIRTUIN抑制后急性热冲击后瓣膜Gape活性的显着降低，但不在任何其他适应组中。出乎意料地，单独的热休克仅降低贻贝的间隙率，其仅适应高食物和高偏振温度，而无需通过热休克和SIRTUIN抑制任何额外的效果。这些研究结果在一起表明，在恢复从热休克后，对低于食物促进较低的热休克期间，SIRTUIN在有机体水平上的重要作用。

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