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首页> 外文期刊>Journal of food engineering >Electrically-assisted Extraction Of Bio-products Using High Pressure Disruption Of Yeast Cells (saccharomyces Cerevisiae)
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Electrically-assisted Extraction Of Bio-products Using High Pressure Disruption Of Yeast Cells (saccharomyces Cerevisiae)

机译：高压破坏酵母细胞（酿酒酵母）的电辅助提取生物产品

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摘要

The efficiency of combined electrically-assisted high pressure homogenization techniques for disruption of wine yeast cells (Saccharomyces cerevisiae bayanus, strain DV10) in an aqueous suspension (1% w/w) was studied. The pulsed electric field (PEF) and high voltage electric discharge (HVED) were applied at electric field strengths of 10kV/cm and 40kV/cm, respectively. The high-pressure homogenization (HPH) technique was applied within pressure range 30-200 MPa at different number of passes (1-20). Releasing of intracellular ionic content was characterized by conductivity disintegration index Z (0-1), releasing of high molecular weight content was analyzed using spectroscopy and electrophoresis methods. It was shown that releasing efficiency of ionic components, enzymes, proteins and other bio-products dramatically depended on the applied method of disruption. Extraction efficiency was rather high for ionic components and small for high molecular weight components in PEF or HVED pre-treated suspensions. However, application of the combined HVED-HPH technique resulted in material loss of integrity by yeast cell walls. Even small disintegration (Z_i = 0.15 ± 0.05) initially induced by HVED pre-treatment resulted in noticeable acceleration of HPH disruption kinetics. The proposed combined HVED-HPH technique may have a good potential in future biotechnological and food applications.
机译：研究了在水悬浮液（1％w / w）中组合的电辅助高压均质技术对葡萄酒酵母细胞（酿酒酵母，Bayanus菌株，DV10株）的破坏效率。分别以10kV / cm和40kV / cm的电场强度施加脉冲电场（PEF）和高压放电（HVED）。在30-200 MPa的压力范围内以不同的通过次数（1-20）应用高压均质化（HPH）技术。通过电导崩解指数Z（0-1）来表征细胞内离子含量的释放，使用光谱学和电泳方法分析了高分子量含量的释放。结果表明，离子成分，酶，蛋白质和其他生物产物的释放效率极大地取决于所应用的破坏方法。 PEF或HVED预处理悬浮液中离子组分的萃取效率相当高，而高分子量组分的萃取效率却很小。但是，结合使用HVED-HPH技术会导致酵母细胞壁的材料完整性丧失。 HVED预处理最初引起的即使很小的崩解（Z_i = 0.15±0.05）也导致HPH破坏动力学的明显加速。拟议的HVED-HPH组合技术在未来的生物技术和食品应用中可能具有良好的潜力。

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