...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Food Science >Construction of Listeria monocytogenes Mutants with In-Frame Deletions in the Phosphotransferase Transport System (PTS) and Analysis of Their Growth under Stress Conditions
【24h】

Construction of Listeria monocytogenes Mutants with In-Frame Deletions in the Phosphotransferase Transport System (PTS) and Analysis of Their Growth under Stress Conditions

机译：磷酸转移酶转运系统（PTS）中框内缺失的单核细胞增生李斯特菌突变体的构建及其在胁迫条件下的生长分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Listeria monocytogenes is a foodborne pathogen that is difficult to eliminate due to its ability to survive under different stress conditions such as low pH and high salt. To better control this pathogen in food, it is important to understand its survival mechanisms under these stress conditions. LMOf2365_0442, 0443, and 0444 encode for phosphotransferase transport system (PTS) permease (fructose-specific IIABC components) that is responsible for sugar transport. LMOf2365_0445 encodes for glycosyl hydrolase. These genes were induced by high pressure and inhibited under salt treatments; therefore, we hypothesized that genes encoding these PTS proteins may be involved in general stress responses. To study the function of these genes, deletion mutants of the PTS genes (LMOf2365_0442, LMOf2365J0443, and LMOf2365_0444) and the downstream gene LMOf2365_0445 were created in L. monocytogenes strain F2365. These deletion mutants were tested under different stress conditions. The growth of ALMOf2365_0445 was increased under nisin (125 μg/mL) treatments compared to the wild-type (P ＜ 0.01). The growth of ALMOf2365_0442 in salt (brain-heart infusion medium with 5% NaCl) was significantly increased (P ＜ 0.01), and ALMOf2365_0442 showed increased growth under acidic conditions (pH 5.0) compared to the wild-type (P ＜ 0.01). The results from phenotypic arrays demonstrated that some of these mutants showed slightly slower growth under different carbon sources and basic conditions. The results indicate that deletion mutants ALMOf2365_0442 and ALMOf2365_0445 were more resistant to multiple stress conditions compared to the wild-type, suggesting that they may contribute to the general stress response in L. monocytogenes. An understanding of the growth of these mutants under multiple stress conditions may assist in the development of intervention strategies to control L. monocytogenes in food.
机译：单核细胞增生李斯特菌是一种食源性病原体，由于其能够在不同的胁迫条件（例如低pH和高盐）下生存而难以消除。为了更好地控制食品中的这种病原体，重要的是要了解其在这些压力条件下的存活机制。 LMOf2365_0442、0443和0444编码负责糖转运的磷酸转移酶转运系统（PTS）渗透酶（果糖特定的IIABC组分）。 LMOf2365_0445编码糖基水解酶。这些基因被高压诱导并在盐处理下被抑制。因此，我们假设编码这些PTS蛋白的基因可能参与了一般应激反应。为了研究这些基因的功能，在单核细胞增生李斯特氏菌菌株F2365中创建了PTS基因的缺失突变体（LMOf2365_0442，LMOf2365J0443和LMOf2365_0444）和下游基因LMOf2365_0445。在不同的胁迫条件下测试了这些缺失突变体。乳链菌肽（125μg/ mL）处理后，ALMOf2365_0445的生长比野生型增加（P ＜0.01）。盐（含5％NaCl的脑-心脏输注介质）中的ALMOf2365_0442的生长显着增加（P ＜0.01），而在酸性条件下（pH 5.0），ALMOf2365_0442的生长比野生型（P ＜0.01）增加。表型阵列的结果表明，这些突变体中的一些在不同的碳源和碱性条件下显示出稍慢的生长。结果表明，与野生型相比，缺失突变体ALMOf2365_0442和ALMOf2365_0445对多种胁迫条件具有更强的抵抗力，表明它们可能对单核细胞增生李斯特菌的一般胁迫反应有所贡献。对这些突变体在多种胁迫条件下的生长的了解可能有助于控制食品中单核细胞增生李斯特菌的干预策略的发展。

著录项

  • 来源
    《Journal of Food Science》 |2013年第9期|1392-1398|共7页
  • 作者

    Yanhong Liu; Marina Ceruso; Yuji Jiang; Atin R. Datta; Laurenda Carter; Errol Strain; Tiziana Pepe; Aniello Anastasi; Pina Fratamico;

  • 作者单位

    Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, 600 East Mermaid Lane, Wyndmoor PA 19038, U.S.A.;

    Dipto. di Scienze Zootecniche e Ispezione degli Alimenti, Sezione Ispezione, Facolta di Medicina Veterinaria, Univ. degli Studi di Napoli Federico II, Via Delpino 1, Naples 80137;

    College of Food Science, Fujian Agriculture and Forestry Univ., Fuzhou, Fujian 3, China 50002;

    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel MD 20708, U.S.A.;

    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel MD 20708, U.S.A.;

    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel MD 20708, U.S.A.;

    Dipto. di Scienze Zootecniche e Ispezione degli Alimenti, Sezione Ispezione, Facolta di Medicina Veterinaria, Univ. degli Studi di Napoli Federico II, Via Delpino 1, Naples 80137;

    Dipto. di Scienze Zootecniche e Ispezione degli Alimenti, Sezione Ispezione, Facolta di Medicina Veterinaria, Univ. degli Studi di Napoli Federico II, Via Delpino 1, Naples 80137;

    Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, 600 East Mermaid Lane, Wyndmoor PA 19038, U.S.A.;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Listeria monocytogenes; phosphotransferase transport system (PTS); stress response;

    机译：李斯特菌;磷酸转移酶转运系统（PTS）;压力反应;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号