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首页> 外文期刊>Journal of Food Science >Rapid Analysis of Glucose, Fructose, Sucrose, and Maltose in Honeys from Different Geographic Regions using Fourier Transform Infrared Spectroscopy and Multivariate Analysis
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Rapid Analysis of Glucose, Fructose, Sucrose, and Maltose in Honeys from Different Geographic Regions using Fourier Transform Infrared Spectroscopy and Multivariate Analysis

机译:使用傅立叶变换红外光谱法和多元分析法快速分析不同地理区域的蜂蜜中的葡萄糖,果糖,蔗糖和麦芽糖

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摘要

Quantitative analysis of glucose, fructose, sucrose, and maltose in different geographic origin honey samples in the world using the Fourier transform infrared (FTIR) spectroscopy and chemometrics such as partial least squares (PLS) and principal component regression was studied. The calibration series consisted of 45 standard mixtures, which were made up of glucose, fructose, sucrose, and maltose. There were distinct peak variations of all sugar mixtures in the spectral "fingerprint" region hetween 1500 and 800 cm~(-1). The calibration model was successfully validated using 7 synthetic blend sets of sugars. The PLS 2nd-derivative model showed the highest degree of prediction accuracy with a highest If value of 0.999. Along with the canonical variate analysis, the calibration model further validated by high-performance liquid chromatography measurements for commercial honey samples demonstrates that FTIR can qualitatively and quantitatively determine the presence of glucose, fructose, sucrose, and maltose in multiple regional honey samples.
机译:使用傅里叶变换红外(FTIR)光谱和化学计量学(例如偏最小二乘(PLS))和主成分回归,研究了世界上不同地理来源的蜂蜜样品中葡萄糖,果糖,蔗糖和麦芽糖的定量分析。校准系列由45种标准混合物组成,这些混合物由葡萄糖,果糖,蔗糖和麦芽糖组成。在1500至800 cm〜(-1)的光谱“指纹”区域中,所有糖混合物均存在明显的峰变化。使用7组合成糖混合液成功验证了校准模型。 PLS二阶导数模型显示出最高的预测准确度,其中If值为0.999。结合规范变量分析,通过高效液相色谱法对商业蜂蜜样品进行进一步验证的校准模型表明,FTIR可以定性和定量确定多个区域蜂蜜样品中葡萄糖,果糖,蔗糖和麦芽糖的存在。

著录项

  • 来源
    《Journal of Food Science》 |2010年第2期|C208-C214|共7页
  • 作者单位

    Dept. of Molecular Biosciences and Bioengineering, Univ. of Hawaii of Manoa, Honolulu, HI, 96822, U.S.A.;

    CTS Foundation, 3081G Paty Drive, Honolulu, HI, 96822, U.S.A.;

    Dept. of Human Nutrition, Food and Animal Science, Univ. of Hawaii of Manoa, Honolulu, HI, 96822, U.S.A.;

    Product Development and Services, Hawaii Agriculture Research Center, Kunia, HI 96759, U.S.A.;

    Dept. of Molecular Biosciences and Bioengineering, Univ. of Hawaii of Manoa, Honolulu, HI, 96822, U.S.A.;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    FTIR; honey authentication; quality; sugars;

    机译:FTIR;蜂蜜认证;质量;糖类;

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