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首页> 外文期刊>Journal of Forestry Research >Genetic variation and division of Pinus sylvestris provenances by ISSR markers
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Genetic variation and division of Pinus sylvestris provenances by ISSR markers

机译：ISSR标记对樟子松种源的遗传变异和划分

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Inter Simple Sequence Repeat (ISSR) was used to detect genetic variation among nine provenances, including 135 individuals. A total of 108 loci were amplified using 10 random primers. The differentiation of the percentage of polymorphic bands (PPB) among different provenances was evident, ranging from 27% to 54%, of which Honghuaerji provenance had the highest PPB and Kalunshan provenance had the lowest PPB. Shannon's Information index (I) at species level was 0.1581 and Nei's gene diversity (h) was 0.2393. Coefficient of gene differentiation (G_(ST)) calculated by Popgene was 0.3965, these results indicated that majority of genetic variation (60.35%) was found within provenances. According to dendrogram among pinus sylvestriv provenances, nine provenances were divided into two provenance areas, namely Daxing'an and Xiaoxing'an Mountains provenance area and Hulunbeier provenance area.
机译：间简单序列重复（ISSR）用于检测包括135个个体在内的9个种源之间的遗传变异。使用10个随机引物扩增了总共108个基因座。不同来源的多态性带（PPB）的百分比差异明显，范围在27％至54％之间，其中红花儿基来源的PPB最高，而卡伦山来源的PPB最低。香农在物种层面的信息指数（I）为0.1581，而Nei的基因多样性（h）为0.2393。 Popgene计算的基因分化系数（G_（ST））为0.3965，这些结果表明在种源内发现了大多数遗传变异（60.35％）。根据樟子松种源间的树状图，将九种种源划分为两个大麦种源区，分别是大兴安和小兴安山种源区和呼伦贝尔种源区。

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