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首页> 外文期刊>Journal of Forestry Research >Cloning and sequence analysis of arom gene from Sclerotinia sclerotiorum
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Cloning and sequence analysis of arom gene from Sclerotinia sclerotiorum

机译：菌核盘菌中Arom基因的克隆与序列分析

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摘要

An arom gene was cloned from genomic DNA of Scleortinia sclerotiorum by inverse PCR. The evolutionary relationships of S. sclerotiorum and other fungi in arom gene were studied. Results showed that the arom gene from of 5. sclerotiorum has a single open reading frame of 4 773 bp and does not include any introns. The derived amino acid sequence consists of 1 590 residues, and it is homologous to all fungal AROM proteins studied so far. The theoretical isoelectric point (pI) and molecular weight (Mw) is 6.5 and 172.66 kD, respectively. GC percentage of the arom gene is 44.94. According to the results of searching from CDD and Prosite database, AROM protein of S. sclerotiorum contains five conserve domains: 3-dehydroquinate synthase domain, 3-dehydroquinate dehydratase (3-dehydroquinase) domain, shikimate 5-dehydrogenase domain, shikimate kinase domain, and -enolpyruvylshikimate-3-phosphate synthase (EPSP sythase) domain, and four motifs: two EPSP synthase signatures, dehydroquinase class I active site, shikimate kinase signature. According to the PIR Site Rule PIRSR000514-1, four functionally important amino acid residues are found by alignment. Putative TATA box and CAAT box locate separately in -23 and -77 loci in 5' un-translated region, and two loci found in downstream arom gene are likely polyadenylation signals. In addition, phylogeny of arom gene is analyzed.
机译：通过反向PCR从菌核菌的基因组DNA中克隆了一个arom基因。研究了sarorotiorum和其他真菌在arom基因中的进化关系。结果显示，来自核盘菌5.的arom基因具有4 773 bp的单个开放阅读框，并且不包含任何内含子。衍生的氨基酸序列由1 590个残基组成，与迄​​今为止研究的所有真菌AROM蛋白同源。理论等电点（pI）和分子量（Mw）分别为6.5和172.66 kD。 arom基因的GC百分比是44.94。根据CDD和Prosite数据库的搜索结果，S。sclerotiorum的AROM蛋白包含五个保守结构域：3-脱氢奎宁酸合酶结构域，3-脱氢奎宁酸脱水酶（3-dehydroquinase）结构域，sh草酸5-dehydrogenase结构域，shikimate激酶结构域，和-enolpyruvylshikimate-3-磷酸盐合酶（EPSP合酶）域，和四个主题：两个EPSP合酶签名，脱氢喹啉类I活性位点，iki草酸激酶签名。根据PIR网站规则PIRSR000514-1，通过比对发现了四个功能上重要的氨基酸残基。推定的TATA框和CAAT框分别位于5'非翻译区的-23和-77位点，在下游arom基因中发现的两个位点可能是聚腺苷酸化信号。另外，分析了arom基因的系统发育。

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