...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of great lakes research >Prevalence of Pathogenic Escherichia coli in Recreational Waters
【24h】

Prevalence of Pathogenic Escherichia coli in Recreational Waters

机译：娱乐水域中致病性大肠杆菌的患病率

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The prevalence of pathogenic Escherichia coli in recreational waters has rarely been quantified. Standard enumerative methods do not identify these pathogens, and thus may not accurately assess the health risk associated with recreational water. A collection of E. coli isolated from bathing waters and adjacent drainage ditches of Maumee Bay State Park (MBSP) on the southwest shore of Lake Erie was examined for the presence of virulence genes characteristic of enterohemorrhagic (EHEC), enteropathogenic (EPEC), and enterotoxigenic (ETEC) E. coli pathogens. The collection, 472 isolates, was screened by DNA-DNA hybridization using gene fragments of heat-labile toxin (Ita, ETEC), heat-stable toxin (sta, ETEC), intimin (eaeA, EHEC, and EPEC) and enterohemolysin (hlyA, EHEC). Hybridization results were confirmed by polymerase chain reaction. Four isolates were shown to carry eaeA. The results indicate that E. coli containing virulence genes do exist as a fraction of the E. coli population at MBSP.
机译：娱乐水域中致病性大肠杆菌的发病率很少被量化。标准枚举方法无法识别这些病原体，因此可能无法准确评估与娱乐用水相关的健康风险。检查了从伊利湖西南岸的莫米湾州立公园（MBE）的沐浴水和相邻排水沟中分离的大肠杆菌集合中是否存在肠出血性（EHEC），肠致病性（EPEC）和产肠毒素（ETEC）的大肠杆菌病原体。使用热不稳定毒素（Ita，ETEC），热稳定毒素（sta，ETEC），内膜素（eaeA，EHEC和EPEC）和肠溶血素（hlyA）的基因片段通过DNA-DNA杂交筛选472个分离物，EHEC）。通过聚合酶链反应证实了杂交结果。显示四个分离株携带eaeA。结果表明，含有毒性基因的大肠杆菌确实作为MBSP大肠杆菌种群的一部分而存在。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号