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首页> 外文期刊>Journal of Harbin Institute of Technology >Carbendazim resistance and calculation effective concentration of carbendazim for Trichoderma harzianum
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Carbendazim resistance and calculation effective concentration of carbendazim for Trichoderma harzianum

机译:哈茨木霉菌的多菌灵耐药性和多菌灵的有效浓度计算

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摘要

There is a method for investigating the transformation of resistance gene of carbendazim into Trichoderma harzianum. In order to introduce the resistance to benzimidazole fungicide into bio-control microorganism Trichoderma harzianum was transformed with the resistance gene. In this study, we investigate resistance level and calculate EC 50 (effective concentration of carbendazim that can survive 50 percent of Trichoderma harzianum in that concentration) and stability of the resistance for the transformant isolate of Trichoderma harzianum. Results show the transformants can growth on the medium containing more than 1 000 mu g/ml carbendazim and the resistance is stabled after 10 times transfer on non-selective medium and have EC 50 average about, 1 200 mu g/ml
机译:有一种研究多菌灵抗性基因向哈茨木霉转化的方法。为了将对苯并咪唑杀真菌剂的抗性引入生物防治微生物中,用抗性基因转化哈茨木霉。在这项研究中,我们调查了抗性水平,并计算了EC 50(在该浓度下能在50%的哈茨木霉中存活的多菌灵的有效浓度)和哈茨木霉转化株的抗性稳定性。结果表明,转化子可以在含有多于1000μg / ml多菌灵的培养基上生长,并且在非选择性培养基上转移10次后,抗性稳定,平均EC 50约为1200μg / ml。

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