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首页> 外文期刊>Journal of Hazardous Materials >Synthesis of biosurfactant stabilized silver nanoparticles, characterization and their potential application for bactericidal purposes
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Synthesis of biosurfactant stabilized silver nanoparticles, characterization and their potential application for bactericidal purposes

机译：生物表面活性剂稳定的银纳米粒子的合成，表征及其施用施用杀菌目的

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摘要

Uniformly dispersed silver nanoparticles (AgNPs) with remarkable colloidal stability were synthesised using chemical reduction method in lipopeptide biosurfactant reverse micelles. Transmission Electron microscopy (TEM), Scanning electron microscopy (SEM) and UV-vis spectroscopy analysis exhibited monodisperse nanoparticles with spherical morphology of diameter of 21 +/- 2. The lipopeptide stabilized AgNPs displayed remarkable antibacterial activity with minimum inhibitory concentration (MIC) value of 15.625 mu g/mL against Gram-negative Pseudomonas aeruginosa CB1 and Gram-positive Bacillus subtilis CN2 strains with a significant dose-dependent reduction of cell viability and loss of membrane integrity. Investigation of AgNPs internalization and dissolution assays demonstrated 42-fold higher leaching of the lipopeptide-stabilized AgNPs compared to the bare AgNPs, and concentration dependent increase in cellular uptake with subsequent damage to intracellular organelles. Further ultrastructural observation using TEM revealed internalization and strong binding of considerable amount of AgNPs on the lipopolysaccharide layer of the Gram-negative and peptidoglycans layer of Gram-positive bacteria indiscriminately, demonstrating robust antibacterial activity and potential application to treat multidrug resistant bacteria.
机译：使用脂肽生物活性剂反向胶束的化学还原方法合成具有显着胶体稳定性的均匀分散的银纳米颗粒（AgNP）。透射电子显微镜（TEM），扫描电子显微镜（SEM）和UV-VIS光谱分析显示出直径21 +/-2的球形形态的单分散纳米颗粒。脂肽稳定的AgNPS显示出显着的抗菌活性，具有最小抑制浓度（MIC）值抗革兰阴性假单胞菌铜绿假单胞菌CB1和革兰氏阳性芽孢杆菌CN2菌株的15.625μg/ ml，具有显着的剂量依赖性降低细胞活力和膜完整性的丧失。与裸酰酰氯相比，AgNP内化和溶解测定的研究表明，脂肽稳定的AgNP的浸出42倍，浓度依赖性增加，细胞摄取随后对细胞内细胞器的损伤。使用TEM的进一步超微结构观察揭示了革兰氏阴性细菌革兰氏阳性细菌的脂多糖层上大量agnps的内化和强大的结合，证明了鲁棒抗菌活性和潜在的应用来治疗多药抗性细菌。

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