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首页> 外文期刊>Journal of Hazardous Materials >Pectin lyase enhances cotton resistance to Verticillium wilt by inducing cell apoptosis of Verticillium dahliae
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Pectin lyase enhances cotton resistance to Verticillium wilt by inducing cell apoptosis of Verticillium dahliae

机译：果胶裂解酶通过诱导维生素的细胞凋亡来增强棉花抗性枯萎病

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摘要

Verticillium wilt caused by Verticillium dahliae Kleb. is a major disease in cotton. We found that pectin lyase can enhance cotton resistance to Verticillium wilt and induce cell apoptosis of V. dahliae strain Vd080. The biocontrol effect of pectin lyase on Vd080 reached 61.9%. Pectin lyase increased ERG4 (Delta (24 (24 (1)))-sterol reductase) expression, the ergosterol content of the cell membrane, the collapse of mitochondrial membrane potential, hydrogen peroxide content, metacaspase activity, and Ca2+ content in the cytoplasm in the Vd080 strain and induced endoplasmic reticulum (ER) stress. Pectin lyase also increased the expression levels of the ER molecular chaperone glucose regulating protein Grp78 (BiP), protein disulfide isomerase (PDI) and calnexin (CNX), reduced the expression levels of the protein Hsp40. When the PDI and BiP genes of Vd080 were knocked out, the mutants square BiP and Delta PDI had reduced sensitivity to pectin lyase. In the absence of external stress, ER stress appeared in mutant Delta BiP cells. Pectin lyase affects the ergosterol content of the Vd080 cell membrane, which causes ER stress and increases the level of Delta BiP to induce Vd080 cell apoptosis. These results demonstrate that pectin lyase can be used to control Verticillium wilt in cotton.
机译：黄萎病枯萎病枯萎病。是棉花的主要疾病。我们发现果胶裂解酶可以增强棉花抗性枯萎病，诱导V.Dahliae菌株VD080的细胞凋亡。果胶裂解酶对VD080上的生物控制作用达到61.9％。果胶裂解酶增加ERG4（δ（24（24）） - 甾醇还原酶）表达，细胞膜的Ergosterol含量，线粒体膜电位塌陷，过氧化氢含量，Metacaspase活性和Ca2 +含量在细胞质中VD080菌株和诱导内质网（ER）应力。果胶裂解酶还增加了ER分子伴侣葡萄糖调节蛋白GRP78（BIP），蛋白二硫化物异构酶（PDI）和CALNEXIN（CNX）的表达水平，降低了蛋白质HSP40的表达水平。当VD080的PDI和BIP基因被敲出时，突变体平方BIP和DELTA PDI对果胶裂解酶的敏感性降低。在没有外部应激的情况下，ER应激出现在突变δBIP细胞中。果胶裂解酶影响VD080细胞膜的Ergoster溶胶含量，从而导致ER应激并增加Delta BIP的水平诱导VD080细胞凋亡。这些结果表明，果胶裂解酶可用于控制棉花的黄萎病。

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  • 来源
    《Journal of Hazardous Materials》 |2021年第2期|124029.1-124029.13|共13页
  • 作者

    Zhang Jing; Yu Xinru; Zhang Chaojun; Zhang Qiong; Sun Ying; Zhu Heqin; Tang Canming;

  • 作者单位

    Nanjing Agr Univ Coll Agron State Key Lab Crop Genet & Germplasm Enhancement Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Agron State Key Lab Crop Genet & Germplasm Enhancement Nanjing 210095 Jiangsu Peoples R China;

    CAAS State Key Lab Cotton Biol Inst Cotton Res Anyang Henan Peoples R China;

    Nanjing Agr Univ Coll Agron State Key Lab Crop Genet & Germplasm Enhancement Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Agron State Key Lab Crop Genet & Germplasm Enhancement Nanjing 210095 Jiangsu Peoples R China;

    CAAS State Key Lab Cotton Biol Inst Cotton Res Anyang Henan Peoples R China;

    Nanjing Agr Univ Coll Agron State Key Lab Crop Genet & Germplasm Enhancement Nanjing 210095 Jiangsu Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cotton; Verticillium dahliae; Pectin lyase; Ergosterol; Endoplasmic reticulum; Apoptosis;

    机译：棉花;果胶;果胶裂解酶;Ergosterol;内质网;细胞凋亡;

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