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首页> 外文期刊>Journal of Hazardous Materials >A miniaturized electrochemical toxicity biosensor based on graphene oxide quantum dots/carboxylated carbon nanotubes for assessment of priority pollutants
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A miniaturized electrochemical toxicity biosensor based on graphene oxide quantum dots/carboxylated carbon nanotubes for assessment of priority pollutants

机译：基于氧化石墨烯量子点/羧化碳纳米管的小型电化学毒性生物传感器，用于评估优先污染物

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摘要

The study presented a sensitive and miniaturized cell-based electrochemical biosensor to assess the toxicity of priority pollutants in the aquatic environment. Human hepatoma (HepG2) cells were used as the biological recognition agent to measure the changes of electrochemical signals and reflect the cell viability. The graphene oxide quantum dots/carboxylated carbon nanotubes hybrid was developed in a facile and green way. Based on the hybrid composite modified pencil graphite electrode, the cell culture and detection vessel was miniaturized to a 96-well plate instead of the traditional culture dish. In addition, three sensitive electrochemical signals attributed to guanine/xanthine, adenine, and hypoxanthine were detected simultaneously. The biosensor was used to evaluate the toxicity of six priority pollutants, including Cd, Hg, Pb, 2,4-dinitrophenol, 2,4,6-trichlorophenol, and pentachlorophenol. The 24h IC50 values obtained by the electrochemical biosensor were lower than those of conventional MTT assay, suggesting the enhanced sensitivity of the electrochemical assay towards heavy metals and phenols. This platform enables the label-free and sensitive detection of cell physiological status with multi-parameters and constitutes a promising approach for toxicity detection of pollutants. It makes possible for automatical and high-throughput analysis on nucleotide catabolism, which may be critical for life science and toxicology. (C) 2016 Elsevier B.V. All rights reserved.
机译：该研究提出了一种灵敏且基于细胞的微型电化学生物传感器，用于评估水生环境中优先污染物的毒性。人类肝癌细胞（HepG2）被用作生物识别剂，以测量电化学信号的变化并反映细胞的活力。氧化石墨烯量子点/羧化碳纳米管杂化物以简便且绿色的方式开发。基于混合复合改性铅笔石墨电极，将细胞培养和检测容器小型化为96孔板，而不是传统的培养皿。此外，同时检测到归因于鸟嘌呤/黄嘌呤，腺嘌呤和次黄嘌呤的三个敏感电化学信号。该生物传感器用于评估六种优先污染物的毒性，包括Cd，Hg，Pb，2,4-二硝基苯酚，2,4,6-三氯苯酚和五氯苯酚。通过电化学生物传感器获得的24h IC50值低于常规MTT分析的IC50值，表明电化学分析对重金属和酚的敏感性增强。该平台可对多种参数进行无标记且灵敏的细胞生理状态检测，构成了污染物毒性检测的有前途的方法。它使核苷酸分解代谢的自动和高通量分析成为可能，这对生命科学和毒理学可能至关重要。 （C）2016 Elsevier B.V.保留所有权利。

著录项

  • 来源
    《Journal of Hazardous Materials》 |2017年第ptab期|272-280|共9页
  • 作者

    Zhu Xiaolin; Wu Guanlan; Lu Nan; Yuan Xing; Li Baikun;

  • 作者单位

    Northeast Normal Univ, Sch Environm, Changchun 130117, Peoples R China;

    Northeast Normal Univ, Sch Environm, Changchun 130117, Peoples R China;

    Northeast Normal Univ, Sch Environm, Changchun 130117, Peoples R China;

    Northeast Normal Univ, Sch Environm, Changchun 130117, Peoples R China;

    Univ Connecticut, Dept Civil & Environm Engn, Storrs, CT 06269 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Priority pollutants; Cell sensor; Toxicity; Graphene oxide quantum dots; Carbon nanotubes;

    机译：优先污染物;电池传感器;毒性;氧化石墨烯量子点;碳纳米管;

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