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首页> 外文期刊>Journal of Hazardous Materials >Electrochemical estrogen screen method based on the electrochemical behavior of MCF-7 cells
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Electrochemical estrogen screen method based on the electrochemical behavior of MCF-7 cells

机译：基于MCF-7细胞电化学行为的电化学雌激素筛选方法

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摘要

It was an urgent task to develop quick, cheap and accurate estrogen screen method for evaluating the estrogen effect of the booming chemicals. In this study, the voltammetric behavior between the estrogen free and normal fragmented MCF-7 cell suspensions were compared, and the electrochemical signal (about 0.68 V attributed by xanthine and guanine) of the estrogen-free fragmented MCF-7 cell suspension was obviously lower than that of the normal one. The electrochemistry detection of ex-secretion purines showed that the ability of ex-secretion purines of cells sharp decreased due to the removing of endogenous estrogen. The results indicated that the electrochemical signal of MCF-7 cells was related to the level of intracellular estrogen. When the level of intracellular estrogen was down-regulated, the concentrations of the xanthine and hypoxanthine decreased, which led to the electrochemical signal of MCF-7 cells fall. Based on the electrochemical signal, the electrochemical estrogen screen method was established. The estrogen effect of estradiol, nonylphenol and bisphenol A was evaluated with the electrochemical method, and the result was accordant with that of MTT assay. The electrochemical estrogen screen method was simple, quickly, cheap, objective, and it exploits a new way for the evaluation of estrogenic effects of chemicals. (C) 2016 Published by Elsevier B.V.
机译：开发快速，廉价，准确的雌激素筛选方法以评估新兴化学品的雌激素作用是当务之急。在这项研究中，比较了无雌激素的片段化MCF-7细胞悬液的伏安行为，无雌激素的片段化MCF-7细胞悬液的电化学信号（黄嘌呤和鸟嘌呤约为0.68 V）明显更低比普通的分泌型嘌呤的电化学检测表明，由于内源性雌激素的去除，细胞分泌型嘌呤的能力急剧下降。结果表明，MCF-7细胞的电化学信号与细胞内雌激素水平有关。当细胞内雌激素水平下调时，黄嘌呤和次黄嘌呤的浓度降低，导致MCF-7细胞的电化学信号下降。基于电化学信号，建立了电化学雌激素筛选方法。用电化学方法评价了雌二醇，壬基酚和双酚A的雌激素作用，结果与MTT法相吻合。电化学雌激素筛选方法简便，快速，廉价，客观，为评价化学物质的雌激素作用开辟了新途径。 （C）2016由Elsevier B.V.发布

著录项

  • 来源
    《Journal of Hazardous Materials》 |2016年第5期|238-243|共6页
  • 作者

    Li Jinlian; Song Jia; Bi Sheng; Zhou Shi; Cui Jiwen; Liu Jiguang; Wu Dongmei;

  • 作者单位

    Jiamusi Univ, Coll Pharm, Jiamusi 154007, Peoples R China;

    Jiamusi Univ, Coll Pharm, Jiamusi 154007, Peoples R China;

    Jiamusi Univ, Affiliated Hosp 1, Jiamusi 154007, Peoples R China;

    Jiamusi Univ, Coll Pharm, Jiamusi 154007, Peoples R China;

    Jiamusi Univ, Coll Pharm, Jiamusi 154007, Peoples R China;

    Jiamusi Univ, Coll Stomatol, Jiamusi 154007, Peoples R China;

    Jiamusi Univ, Coll Pharm, Jiamusi 154007, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Estrogen effect; Electrochemistry method; MCF-7 cell; Purine metabolism;

    机译：雌激素作用;电化学方法;MCF-7细胞;嘌呤代谢;

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