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首页> 外文期刊>Journal of Hazardous Materials >Electrochemistry of copper(Ⅱ) induced complexes in mycorrhizal maize plant tissues
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Electrochemistry of copper(Ⅱ) induced complexes in mycorrhizal maize plant tissues

机译：铜（Ⅱ）诱导的菌根玉米植物组织中复合物的电化学

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摘要

Aim of the present paper was to study the electrochemical behavior of copper(Ⅱ) induced complexes in extracts obtained from mycorrhizal and non-mycorrhizal maize (Zea mays L.) plants grown at two concentrations of copper(Ⅱ): physiological (31.7 ng/mL) and toxic (317 μg/mL). Protein content was determined in the plant extracts and, after dilution to proper concentration, various concentrations of copper(Ⅱ) ions (0,100, 200 and 400 μg/mL) were added and incubated for 1 h at 37℃ Further, the extracts were analyzed using flow injection analysis with electrochemical detection. The hydrodynamic voltammogram (HDV), which was obtained for each sample, indicated the complex creation. Steepness of measured dependencies was as follows: control 317 μg/mL of copper < control 31.7 ng/mL of copper < mycorrhizal 31.7 ng/mL of copper < mycorrhizal 317 μg/mL of copper. Based on these results it can be concluded that mycorrhizal fungus actively blocks transport copper(Ⅱ) ions to upper parts of a plant by means of adsorbing of copper(Ⅱ) in roots. Rapid complex formation was determined under applied potentials 300, 500 and 600 mV during the measuring HDVs. It was also verified that mycorrhizal colonization reduced root to shoot translocation of Cu(Ⅱ) ions.
机译：本文的目的是研究在两种浓度的铜（Ⅱ）下生长的菌根和非菌根玉米（Zea mays L.）植物提取物中铜（Ⅱ）诱导的配合物的电化学行为：生理（31.7 ng / ng mL）和有毒物质（317μg/ mL）。测定植物提取物中的蛋白质含量，并稀释至适当浓度后，添加各种浓度的铜（Ⅱ）离子（0,100、200和400μg/ mL）并在37℃下孵育1小时。使用流动注射分析和电化学检测。从每个样品获得的流体动力学伏安图（HDV）表示复杂的创建。测得的依赖性的陡度如下：对照317μg/ mL的铜<对照31.7 ng / mL的铜<菌根31.7 ng / mL的铜<菌根317μg/ mL的铜。从这些结果可以得出结论，菌根真菌通过吸附根中的铜（Ⅱ）而有效地阻止了铜（Ⅱ）离子向植物上部的转运。在测量HDV期间，在施加电势300、500和600 mV的条件下确定了快速的络合物形成。还证实了菌根定植减少了Cu（Ⅱ）离子的根到芽的移位。

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  • 来源
    《Journal of Hazardous Materials》 |2012年第2012期|p.257-263|共7页
  • 作者

    Ondrej Zitka; Miguel-Angel Merlos; Vojtech Adam; Nuria Ferrol; Miroslav Pohanka; Jaromir Hubalek; Josef Zehnalek; Libuse Trnkova; Rene Kizek;

  • 作者单位

    Department of Chemistry and Biochemistry, Zemedelska 1, CZ-613 00 Brno, Czech Republic,Lead and Cadmium lnicitatives, United Nation Environment Program, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic;

    Departamento de Microbiologia del Sueloy Sistemas Simbioticos, Estacidn Experimental delZaidin, CS1C, Profesor Albareda 1, Granada 18008, Spain;

    Department of Chemistry and Biochemistry, Zemedelska 1, CZ-613 00 Brno, Czech Republic,Lead and Cadmium lnicitatives, United Nation Environment Program, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic,Central European institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic;

    Departamento de Microbiologia del Sueloy Sistemas Simbioticos, Estacidn Experimental delZaidin, CS1C, Profesor Albareda 1, Granada 18008, Spain;

    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ-50001 Hradec Kralove, Czech Republic;

    Central European institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic,Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, CZ-616 00 Brno, Czech Republic;

    Department of Chemistry and Biochemistry, Zemedelska 1, CZ-613 00 Brno, Czech Republic;

    Department of Chemistry and Biochemistry, Zemedelska 1, CZ-613 00 Brno, Czech Republic,Lead and Cadmium lnicitatives, United Nation Environment Program, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic,Central European institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic, Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic;

    Department of Chemistry and Biochemistry, Zemedelska 1, CZ-613 00 Brno, Czech Republic,Lead and Cadmium lnicitatives, United Nation Environment Program, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic,Central European institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    plant; mycorrhizal fungi; copper; flow injection analysis with; electrochemical detection; hydrodynamic voltammogram;

    机译：厂;菌根真菌铜;流动注射分析;电化学检测流体动力学伏安图;

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