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首页> 外文期刊>Journal of Hazardous Materials >Organochlorine pesticide, endosulfan induced cellular and organismal response in Drosophila melanogaster
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Organochlorine pesticide, endosulfan induced cellular and organismal response in Drosophila melanogaster

机译：有机氯农药，硫丹在果蝇中诱导的细胞和生物反应

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摘要

The effect of endosulfan (0.02-2.0 μgmL~(-1) to Drosophila melanogaster (Oregon R~+) at the cellular and organismal levels was examined. Third instar larvae of D. melanogaster and the strains transgenic for hsp70, hsp83 and hsp26 were exposed to endosulfan through food for 12-48h to examine the heat shock proteins (hsps), reactive oxygen species (ROS) generation, anti-oxidant stress markers and xeno-biotic metabolism enzymes. We observed a concentration- and time-dependent significant induction of only small hsps (hsp23 > hsp22) in the exposed organism in concurrence with a significant induction of ROS generation, oxidative stress and xenobiotic metabolism markers. Sub-organismal response was to be propagated towards organismal response, i.e., delay in the emergence of flies and decreased locomotor behaviour. Organisms with diminished locomotion also exhibited significantly lowered acetyl-cholinesterase activity. A significant positive correlation observed among ROS generation and different cellular endpoints (small hsps, oxidative stress markers, cytochrome P450 activities) in the exposed organism indicate a modulatory role of ROS in endosulfan-mediated cellular toxicity. The study thus suggests that the adverse effects of endosulfan in exposed Drosophila are manifested both at cellular and organismal levels and recommends Drosophila as an alternative animal model for screening the risk caused by environmental chemicals.
机译：研究了硫丹（0.02-2.0μgmL〜（-1）对果蝇果蝇（Oregon R〜+）在细胞和机体水平的影响，研究了D. melanogaster的三龄幼虫以及转基因的hsp70，hsp83和hsp26菌株。通过食物暴露于硫丹中12-48h，以检查热激蛋白（hsps），活性氧（ROS）生成，抗氧化应激标记和异生物代谢酶，我们观察到了浓度和时间依赖性的显着诱导暴露的有机体中只有少量的hsps（hsp23> hsp22）与ROS的产生，氧化应激和异源生物代谢标记物的显着诱导同时发生，亚有机体反应应传播至生物体反应，即延缓果蝇的出现。运动减少的生物也表现出显着降低的乙酰胆碱酯酶活性，在ROS的产生和分化之间存在显着的正相关。暴露生物体中的不同细胞终点（小的hsps，氧化应激标志物，细胞色素P450活性）表明ROS在硫丹介导的细胞毒性中的调节作用。因此，该研究表明硫丹在暴露的果蝇中的不良反应在细胞和机体水平上均得到体现，并建议果蝇作为替代动物模型来筛选环境化学物质引起的风险。

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  • 来源
    《Journal of Hazardous Materials》 |2012年第30期|p.275-287|共13页
  • 作者

    Anurag Sharma; M. Mishra; A.K. Shukla; R. Kumar; M.Z. Abdin; D. Kar Chowdhuri;

  • 作者单位

    Embryotoxicology Section. CSlR-lndian Institute of Toxicology Research (CS1R-11TR). Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh. India;

    Embryotoxicology Section. CSlR-lndian Institute of Toxicology Research (CS1R-11TR). Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh. India;

    Embryotoxicology Section. CSlR-lndian Institute of Toxicology Research (CS1R-11TR). Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh. India;

    Analytical Section. CSIR-lndian Institute of Toxicology Research (CSIR-IITR), Mahatma Gandhi Marg. Lucknow 226001, Uttar Pradesh. India;

    Department of Biotechnology, Jamia Hamdard. New Delhi 110062, India;

    Embryotoxicology Section. CSlR-lndian Institute of Toxicology Research (CS1R-11TR). Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh. India;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    D. melanogaster; heat shock proteins; oxidative stress; DNA damage; organismal response; endosulfan;

    机译：D.黑色素瘤;热激蛋白;氧化应激DNA损伤;机体反应;硫丹;

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