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首页> 外文期刊>Journal of Hazardous Materials >Characterization of the microbial community from the marine sediment of the Venice lagoon capable of reductive dechlorination of coplanar polychlorinated biphenyls (PCBs)
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Characterization of the microbial community from the marine sediment of the Venice lagoon capable of reductive dechlorination of coplanar polychlorinated biphenyls (PCBs)

机译：从威尼斯泻湖的海洋沉积物中表征微生物，能够共平面多氯联苯（PCBs）还原脱氯

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摘要

The native microbial community of a contaminated sediment from Brentella Canal (Venice Lagoon, Italy) was enriched in slurry microcosms consisting of sterile sediment suspended in sterile site water in the presence of 3,3',4,4'-tetrachlorobiphenyl, 3,3',4,4',5- and 2,3',4,4',5-pentachlorobiphenyls, 3,3',4,4',5,5'-and 2,3,3'.4,4',5-hexachlorobiphenyls. The enrichment cultures were characterized at each subculturing step by 16S rRNA gene Terminal-Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE) analysis. About 90% of spiked polychlorinated biphenyls (PCBs) were stoichiometrically converted into di- and tri-chlorinated congeners by each enriched culture via dechlorination of flanked para chlorines and ortho-flanked meta chlorines. A 2-fold increase in PCB-dechlorination rate, the disappearance of lag phase, as well as a remarkable increase of sulfate consumption and a decline of methanogenic activity, were observed throughout subculturing. A reduction of complexity of the archaeal community, which was composed by Methanomicrobiales and Methanosarcinales, was also observed as a result of culture enrichment. The bacterial community included members of the Alpha, Gamma, Delta and Epsilon divisions of Proteobacteria, Firmicutes and Chloroflexi. Two sequence phylotypes related to the genus Sulforovum and the species Desulfococcus multivorans and two Chloroflexi nriched throughout subculturing, thus suggesting that these bacteria were involved in PCB dechlorination in the marine sediments of Brentella canal.
机译：来自Brentella运河（意大利威尼斯泻湖）的受污染沉积物的原生微生物群落富含泥浆微观世界，其中包括在3,3'，4,4'-四氯联苯，3,3存在下悬浮在无菌场地水中的无菌沉积物'，4,4'，5-和2,3'，4,4'，5-五氯联苯，3,3'，4,4'，5,5'-和2,3,3'.4,4 '，5-六氯联苯。在每个传代步骤中，通过16S rRNA基因末端限制性片段长度多态性（T-RFLP）和变性梯度凝胶电泳（DGGE）分析来表征富集培养。每种富集培养物通过对侧氯和邻位间氯的脱氯，将约90％的加标多氯联苯（PCB）化学计量转换为二氯和三氯同类物。在整个传代培养过程中，观察到PCB脱氯速率增加了2倍，滞后相的消失，硫酸盐的消耗显着增加，产甲烷活性降低。由于培养物的丰富化，还观察到由甲甲烷菌和甲烷二甲虫组成的古细菌群落的复杂性降低。细菌群落包括Proteobacteria，Firmicutes和Chloroflexi的Alpha，Gamma，Delta和Epsilon部门的成员。整个亚培养过程中，与Sulforovum属和Desulfococcus multivorans物种相关的两个序列系统型和两个富集的Cloroflexi丰富，因此表明这些细菌参与了Brentella运河海洋沉积物中的PCB脱氯。

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  • 来源
    《Journal of Hazardous Materials》 |2010年第3期|417-426|共10页
  • 作者

    Giulio Zanaroli; Annalisa Balloi; Andrea Negroni; Daniele Daffonchio; Lily Y. Young; Fabio Fava;

  • 作者单位

    DICASM, Faculty of Engineering, University of Bologna, via Terracini 28, Bologna 40131, Italy;

    rnDipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita degli Studi di Milano, via Celoria 2,20133 Milan, Italy;

    rnDICASM, Faculty of Engineering, University of Bologna, via Terracini 28, Bologna 40131, Italy;

    rnDipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita degli Studi di Milano, via Celoria 2,20133 Milan, Italy;

    rnBiotechnology Center for Agriculture and the Environment, Rutgers University, 59 Dudley Road, New Brunswick NJ, USA;

    rnDICASM, Faculty of Engineering, University of Bologna, via Terracini 28, Bologna 40131, Italy ENVIREN (The cluster of environmental laboratories of the Emilia Romagna Region), via Gobetti 101, Bologna 40129, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    polychlorinated biphenyls; reductive dechlorination; marine sediment; T-RFLP; DGGE;

    机译：多氯联苯;还原脱氯海洋沉积物T-RFLP;DGGE;

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