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首页> 外文期刊>Journal of Heat Transfer >Principles of Tissue Engineering With Nonthermal Irreversible Electroporation
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Principles of Tissue Engineering With Nonthermal Irreversible Electroporation

机译：非热不可逆电穿孔的组织工程原理

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摘要

Nonthermal irreversible electroporation (NTIRE) is an emerging tissue ablation modality that may be ideally suited in developing a decellularized tissue graft. NTIRE utilizes short electric pulses that produce nanoscale defects in the cell membrane lipid bilayer. The electric parameters can be chosen in such a way that Joule heating to the tissue is minimized and cell death occurs solely due to loss in cell homeostasis. By coupling NTIRE with the body's response, the cells can be selectively ablated and removed, leaving behind a tissue scaffold. Here, we introduce two different methods for developing a decellularized arterial scaffold. The first uses an electrode clamp that is applied to the outside of a rodent carotid artery and the second applies an endovascular minimally invasive approach to apply electric fields from the inner surface of the blood vessels. Both methods are first modeled using a transient finite element analysis of electric and thermal fields to ensure that the electric parameters used in this study will result in minimal thermal damage. Experimental work demonstrates that both techniques result in not only a decellularized arterial construct but an endothelial regrowth is evident along the lumen 7 days after treatment, indicating that the extracellular matrix was not damaged by electric and thermal fields and is still able to support cell growth.
机译：非热不可逆电穿孔（NTIRE）是一种新兴的组织消融方式，可能非常适合开发脱细胞的组织移植物。 NTIRE利用短电脉冲在细胞膜脂质双层中产生纳米级缺陷。可以选择电参数，以使对组织的焦耳热最小化，并且仅由于细胞稳态损失而发生细胞死亡。通过将NTIRE与机体的反应耦合，可以选择性地消融和去除细胞，留下组织支架。在这里，我们介绍了两种不同的方法来开发脱细胞的动脉支架。第一种使用电极夹钳，该电极夹钳用于啮齿动物的颈动脉的外部，第二种使用血管内微创方法从血管内表面施加电场。两种方法都首先使用电场和热场的瞬态有限元分析来建模，以确保本研究中使用的电参数将导致最小的热损伤。实验工作表明，两种技术不仅导致脱细胞的动脉构造，而且在处理后7天沿管腔也明显出现了内皮再生长，这表明细胞外基质并未受到电场和热场的破坏，并且仍然能够支持细胞生长。

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  • 来源
    《Journal of Heat Transfer》 |2011年第1期|p.011004.1-011004.8|共8页
  • 作者

    Mary Phillips; Elad Maor; Boris Rubinsky;

  • 作者单位

    Department of Mechanical Engineering,University of California, Berkeley,6124 Etcheverry Hall,Berkeley, CA 94720;

    Biophysics Graduate Group,University of California, Berkeley,Berkeley, CA 94720;

    Department of Mechanical Engineering and Biophysics Graduate Group, University of California, Berkeley,Berkeley, CA 94720;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    irreversible electroporation; bioheat transfer; tissue engineering;

    机译：不可逆的电穿孔生物热传递;组织工程;

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