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首页> 外文期刊>Journal of Huazhong University of Science and Technology >Anti-cancer Effects of Deguelin on Human Leukemia K562 and K562/ADM Cells In Vitro
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Anti-cancer Effects of Deguelin on Human Leukemia K562 and K562/ADM Cells In Vitro

机译：德盖林对人白血病K562和K562 / ADM细胞的体外抗癌作用

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摘要

In order to investigate the anti-cancer effects of deguelin and on K562 and K562/ADM cells in vitro and the underlying molecular mechanism and compare the cytotoxicity of deguelin on K562, K562/ADM cells and human peripheral blood mononuclear cells (PBMCs). The effects of deguelin on cell proliferation were assessed by MTT assay. Apoptosis were detected by Annexin Ⅴ/PI double-labeled cytometry. The effects of deguelin on the cell cycle were studied by a propidium iodide method. Our study showed that deguelin inhibited the proliferation of K562 cell and K562/ADM cell in a time- and dose-dependent manner and had minimal effects on normal human peripheral blood mononuclear cells. The ratio of IC_(50) value of deguelin of 24 h on K562/ADM cells to K562 cells was only 1.27, which was significantly lower than the ratio of IC_(50) value of ADM (higher than 20). Deguelin could induce apoptosis of K562 cells and K562/ADM cells. K562 cells were arrested at G_2/M phase while K562/ADM cells were arrested at G_0/G_1 phase. Our results suggested that deguelin was a novel anti-leukemia agents with high efficacy and low toxicity and it is also a promising agent for reversing drug resistance.
机译：为了研究德盖林及其体外对K562和K562 / ADM细胞的抗癌作用及其潜在的分子机制，并比较德盖林对K562，K562 / ADM细胞和人外周血单个核细胞（PBMC）的细胞毒性。通过MTT分析评估了地精蛋白对细胞增殖的影响。通过膜联蛋白Ⅴ/ PI双标记细胞仪检测细胞凋亡。用碘化丙啶法研究了地精蛋白对细胞周期的影响。我们的研究表明，地精蛋白以时间和剂量依赖性的方式抑制K562细胞和K562 / ADM细胞的增殖，并且对正常人外周血单个核细胞的影响最小。 K562 / ADM细胞与K562细胞在24 h的鸟豆蛋白IC_（50）值之比仅为1.27，大大低于ADM的IC_（50）值之比（高于20）。 Deguelin可以诱导K562细胞和K562 / ADM细胞凋亡。 K562细胞停滞在G_2 / M期，而K562 / ADM细胞停滞在G_0 / G_1期。我们的研究结果表明，地精蛋白是一种新型的高效，低毒的抗白血病药，也是逆转耐药性的有前途的药物。

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