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首页> 外文期刊>Journal of Huazhong University of Science and Technology >Expression of Survivin and Its Splice Variants in Gastric Cancer
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Expression of Survivin and Its Splice Variants in Gastric Cancer

机译：Survivin及其剪接变异体在胃癌中的表达

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摘要

Survivin variants specific real time quantitative RT-PCR was developed to analyze their expression in 53 paired cancer and para-cancerous tissues, and the expression of the wild-type survivin protein was detected by immunohistochemistry. The results showed that survivin mRNA and protein were expressed in gastric cancer and para-cancerous tissues. The survivin-2B was dominantly expressed in para-cancerous tissues, whereas the survivin-△EX3 was more frequently detected in cancer tissues. The positive rate of survivin-2a was 100% in both cancer and para-cancerous tissues, but its relative transcript expression level was not significantly increased in cancer tissues in comparison with para-cancerous tissues. The correlation analysis revealed that the expression of survivin-2a mRNA was significantly associated with that of total survivin (r_s=0.4178, P=0.0018), whereas inversely to that of survivin-△EX3 (r_s=-0.4506, P=0.0007). It was suggested that survivin-2a may act as an antagonist of survivin-△EX3. The balance between antiapoptotic survivin iso-forms and nonantiapoptotic ones may play an important role in tumorigenesis and tumor progression. Promising value is hinted to analyze survivin and its variants in tumor early diagnosis and distinguishing malignant tumors from benign ones.
机译：开发了Survivin变体特异性实时定量RT-PCR，以分析其在53个配对的癌和癌旁组织中的表达，并通过免疫组织化学检测野生型survivin蛋白的表达。结果表明，survivin mRNA和蛋白在胃癌和癌旁组织中表达。 survivin-2B主要在癌旁组织中表达，而survivin-△EX3在癌组织中更常见。 survivin-2a在癌症和癌旁组织中的阳性率均为100％，但与癌旁组织相比，其相对转录表达水平在癌组织中并未显着增加。相关分析显示，survivin-2a mRNA的表达与总survivin的表达显着相关（r_s = 0.4178，P = 0.0018），而与survivin-△EX3的表达相反（r_s = -0.4506，P = 0.0007）。提示survivin-2a可以作为survivin-△EX3的拮抗剂。抗凋亡survivin同工型和非抗凋亡survivin同工型之间的平衡可能在肿瘤发生和肿瘤进展中起重要作用。提示在肿瘤的早期诊断中分析survivin及其变异体，将恶性肿瘤与良性肿瘤区分开来具有潜在的价值。

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