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首页> 外文期刊>Journal of Huazhong University of Science and Technology >Rapid Inhibition of the Glutamate-induced Increase of Intracellular Free Calcium by Magnesium in Rat Hippocampal Neurons
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Rapid Inhibition of the Glutamate-induced Increase of Intracellular Free Calcium by Magnesium in Rat Hippocampal Neurons

机译：镁对大鼠海马神经元谷氨酸诱导的细胞内游离钙增加的快速抑制

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摘要

By using Fura-2/AM, the effects of magnesium (Mg~(2+)) on the glutamate-induced increase of intracellular free calcium ([Ca~(2+)]i) in the cultured hippocampal neurons and the features were investigated by integrated photoelectric detecting system. The experiments were designed to three groups (The drug was spit to the cells for 20 s): Group A receiving 1 X 10~(-5) mol/L glutamate; Group B receiving 1 X 10~(-5) mol/L glutamate and 1 X 10~(-5) mol/L Mg~(2+) simultaneously; Group C receiving 1 X 10~(-5) mol/L glutamate again after [Ca~(2+)]i in group B back to the baseline. The results showed that in group A, [Ca~(2+)]i was obviously increased. In group B, the changes in [Ca~(2+)] i and the peak value were significantly decreased. Moreover, the elevation of Phase 1 was slowed down and Phase 2 was shortened to some extent, and the plateau phase between them was relatively prolonged. In group C, calcium oscillation similar to that in group A occurred, but both the Phase 1 and Phase 2 were shortened and the Δ[Ca~(2+)]i was slightly decreased. It was suggested that Mg~(2+) could quickly inhibit the rise of [Ca~(2+)]i induced by glutamate in the cultured hippocampal neurons in rats.
机译：通过使用Fura-2 / AM，镁（Mg〜（2+））对谷氨酸诱导的海马神经元细胞内游离钙（[Ca〜（2 +）] i）增加的影响及其特征由集成光电检测系统进行调查。实验分为三组（将药物吐入细胞20 s）：A组接受1 X 10〜（-5）mol / L谷氨酸； B组同时接受1 X 10〜（-5）mol / L谷氨酸和1 X 10〜（-5）mol / L Mg〜（2+）;在B组的[Ca〜（2 +）] i回到基线后，C组再次接受1 X 10〜（-5）mol / L谷氨酸。结果表明，A组中[Ca〜（2 +）] i明显升高。 B组[Ca〜（2+）] i的变化和峰值均明显降低。此外，阶段1的海拔降低了，阶段2的缩短了一定程度，并且它们之间的平稳期相对延长了。在C组中，发生了与A组相似的钙振荡，但是阶段1和阶段2都缩短了，Δ[Ca〜（2 +）] i略有降低。提示Mg〜（2+）可以快速抑制谷氨酸诱导的大鼠海马神经元[Ca〜（2 +）] i的升高。

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