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The use of atmospheric and room temperature plasma mutagenesis to create a brewing yeast with reduced acetaldehyde production

机译：利用常压和室温血浆诱变产生酿酒酵母，降低乙醛的产生

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摘要

High acetaldehyde levels in beer from yeast metabolism is a major concern for brewers in China. To obtain a strain with lower acetaldehyde production, this work reports a novel approach based on atmospheric and room temperature plasma mutagenesis and high-throughput screening using 4-methylpyrazole + disulphiram plating. A mutant LAL-8a with lower acetaldehyde-producing capability was obtained. The alcohol dehydrogenase activity decreased by 54% compared with the wild-type M14 and the aldehyde dehydrogenase activity increased by 64% of the wild-type strain. Through domestication and fermentation in EBC tubes, the mutant LAL-8a was shown to produce 2.2mg/L acetaldehyde, 88.2% less than the wild-type strain M14. In addition, the ratio of higher esters to alcohols in beer fermented by the mutant LAL-8a (0.28) was higher than M14 (0.16). The fermentation performance of LAL-8a was similar to that of the wild-type M14. This work suggests strain LAL-8a a promising option for the brewing industry. (c) 2018 The Institute of Brewing & Distilling
机译：啤酒中来自酵母新陈代谢的乙醛含量高是中国啤酒生产者的主要关切。为了获得乙醛生成量较低的菌株，这项工作报告了一种基于大气和室温血浆诱变和使用4-甲基吡唑+双硫仑平板进行高通量筛选的新方法。获得具有较低乙醛生产能力的突变体LAL-8a。与野生型M14相比，醇脱氢酶活性降低了54％，醛脱氢酶活性提高了64％。通过驯化和在EBC管中发酵，突变体LAL-8a产生的乙醛为2.2mg / L，比野生型菌株M14低88.2％。另外，通过突变LAL-8a发酵的啤酒中高级酯与醇的比例（0.28）高于M14（0.16）。 LAL-8a的发酵性能与野生型M14相似。这项工作表明，LAL-8a菌株是酿造业的有前途的选择。 （c）2018年酿造与蒸馏研究所

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  • 来源
    《Journal of the Institute of Brewing》 |2018年第3期|236-243|共8页
  • 作者

    Liu Chunfeng; Li Qi; Niu Chengtuo; Tian Yaping; Zhao Yijin; Yin Xiangsheng;

  • 作者单位

    Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Lab Brewing Sci & Engn, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Lab Brewing Sci & Engn, Wuxi 214122, Jiangsu, Peoples R China;

    Rahr Corp, 800 First Ave West, Shakopee, MN 55379 USA;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    acetaldehyde; beer; brewing yeast; ARTP; flavour;

    机译：乙醛;啤酒;发酵酵母;ARTP;风味;

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