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Efficient very-high-gravity fermentation of sugarcane molasses by industrial yeast strains

机译:工业酵母菌株对甘蔗糖蜜的高效超重力发酵

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In this study industrial strains were inoculated, in successive cycles, at high cell density into a sugarcane-based juice containing normal (22%, w/v) and very high sucrose (30%, w/v) levels and supplemented with peptone as a nitrogen source. At 30 degrees C, in shaken cultures, in the normal gravity situation, efficient sucrose utilization occurred in both the supplemented and unsupplemented medium. At higher sugar concentrations, supplementation with peptone induced a more efficient fermentation compared with the unsupplemented medium, with higher biomass accumulation and maintenance of cell viability, and ethanol levels as high as 16% (v/v). Trehalose was also high during the cycles, probably as a necessary response of the yeast to the high stress fermentation conditions. This suggests that it is possible to increase ethanol production by using very-high-fermentation technology and that nutritional supplementation would have a positive effect on the fermentation, allowing for efficient sugar consumption and cell viability maintenance. Copyright (c) 2016 The Institute of Brewing & Distilling
机译:在这项研究中,工业菌株以高细胞密度连续循环接种到甘蔗汁中,该汁含有正常(22%,w / v)和非常高的蔗糖(30%,w​​ / v)水平,并补充有蛋白ept作为氮源。在摇动的培养物中,在30摄氏度下,在正常重力情况下,补充和未补充培养基中都发生了有效的蔗糖利用。在较高的糖浓度下,与未添加的培养基相比,补充蛋白p可诱导更有效的发酵,具有更高的生物量积累和细胞活力维持能力,乙醇含量高达16%(v / v)。海藻糖在循环中也很高,可能是酵母对高压力发酵条件的必要反应。这表明可以通过使用非常高的发酵技术来增加乙醇的产量,并且营养补充将对发酵产生积极影响,从而实现有效的糖消耗和细胞活力的维持。版权所有(c)2016酿酒与蒸馏研究所

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