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首页> 外文期刊>Journal of the Institute of Brewing >Comparison of a new digital imaging technique for yeast cell counting and viability assessments with traditional methods
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Comparison of a new digital imaging technique for yeast cell counting and viability assessments with traditional methods

机译：新型数字成像技术与传统方法的酵母细胞计数和生存能力评估的比较

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In this study, a new rapid automated yeast cell counter was assessed. The cell counter (Aber Countstar) uses bright-field microscopy and a dye-exclusion method. This study's aim was to determine whether this method could be effectively employed in an automated slide based counter to assess viability and to compare this method with results from traditional microscopy and from a radio-frequency impedance-based instrument (Aber Compact Lab Yeast Analyser). Excellent correlations were observed between methods. The instrument performed well over a range of yeast concentrations (R-2 = 0.9913 correlation between automated and manual live cell concentrations). Cell diameters compared well with manual recordings. The instrument was also able to track decreasing cell viability in conjunction with the haemocytometer at viabilities over 20%. The radio-frequency impedance based instrument exhibited the smallest deviation between 10 repeats, followed by the cell counter (SD:+/- 1.08x10(7);+/- 5.97x10(7), respectively). Manual counts using a haemocytometer exhibited the largest error between repeats (SD:+/- 2.63x10(8)) and also required substantially more time (2.28 min) compared with the cell counter (7 sec). The automated cell counter successfully reduced inter-operator errors, a major hindrance with manual analyses. Tests carried out at a brewery in the UK demonstrated that the cell counter provides consistent counts for assorted yeast strains. External tests highlighted the instrument's ease of use and consistency among different strains of brewing yeast and various stages in the brewing process. Copyright (c) 2015 The Institute of Brewing & Distilling
机译：在这项研究中，评估了一种新的快速自动酵母细胞计数器。细胞计数器（Aber Countstar）使用明场显微镜和染料排除法。这项研究的目的是确定这种方法是否可以有效地用于基于幻灯片的自动计数器中，以评估其可行性，并将该方法与传统显微镜和基于射频阻抗的仪器（Aber Compact Lab Yeast Analyser）的结果进行比较。方法之间观察到极好的相关性。该仪器在一定范围的酵母浓度下表现良好（R-2 = 0.9913在自动和手动活细胞浓度之间的相关性）。电池直径与手动记录相比很好。该仪器还能够与血细胞计数器一起跟踪超过20％的活力下降的细胞活力。基于射频阻抗的仪器在10次重复之间显示出最小的偏差，其次是信元计数器（分别为SD：+/- 1.08x10（7）； +/- 5.97x10（7））。与血细胞计数器（7秒）相比，使用血球计数器进行的手动计数显示重复之间的最大误差（SD：+/- 2.63x10（8）），并且还需要更长的时间（2.28分钟）。自动细胞计数仪成功减少了操作员之间的错误，这是人工分析的主要障碍。在英国一家啤酒厂进行的测试表明，该细胞计数器可为各种酵母菌株提供一致的计数。外部测试突出了该仪器的易用性，以及在不同菌株的酿造酵母以及酿造过程的各个阶段之间的一致性。版权所有（c）2015酿酒与蒸馏研究所

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