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首页> 外文期刊>Journal of the Institute of Brewing >A rapid identification of barley varieties using DNA-AFLP
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A rapid identification of barley varieties using DNA-AFLP

机译：利用DNA-AFLP快速鉴定大麦品种

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摘要

Barley (Hordeum vulgare L.) is one of the most ancient crops in the world, and is mainly used in feed, as a grain and as industrial material for beer, medicine and health protection. Its purity can affect beer taste and quality, thus it is important to set up rapid and accurate identification methods for the malting industry. In this study, 27 domestic and imported malt barley varieties were studied. Three pair primers of EcoR I + 3/Mse I + 3 were selected from 64 different primer combinations for DNA-AFLP (amplified fragment length polymorphisms) and an AFLP fingerprinting map of the 27 domestic and imported malt barley varieties was constructed, with the primer combination of E-AGG/M-CAA amplification after polyacrylamide gel electrophoresis. With this fingerprinting map, it was possible to distinguish all of the 27 malt barley varieties with 10 specific loci. This could be useful for the identification of malt barley varieties in the beer industry. Copyright (c) 2015 The Institute of Brewing & Distilling
机译：大麦（Hordeum vulgare L.）是世界上最古老的农作物之一，主要用于饲料，谷物和啤酒，医药和健康保护的工业原料。其纯度会影响啤酒的口感和品质，因此为麦芽行业建立快速，准确的识别方法很重要。在这项研究中，对27种国内和进口的麦芽大麦品种进行了研究。从64种DNA-AFLP引物组合（扩增的片段长度多态性）中选择三对EcoR I + 3 / Mse I + 3引物，并构建了27种国内外麦芽大麦品种的AFLP指纹图谱。聚丙烯酰胺凝胶电泳后E-AGG / M-CAA扩增的组合。使用该指纹图谱，可以区分具有10个特定位点的27种麦芽大麦品种。这对于啤酒工业中麦芽大麦品种的鉴定可能有用。版权所有（c）2015酿酒与蒸馏研究所

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