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Maintaining yeast viability in continuous primary beer fermentation

机译：在连续一次啤酒发酵中保持酵母活力

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摘要

Continuous fermentation is a long known and vastly studied process. The use of immobilized cell technology (ICT) is exploited in a significant number of studies owing to the associated high volumetric productivity, time savings and low capital demand. This work was aimed at solving one of the most relevant obstacles to implementing ICT on a large scale in beer fermentations, namely the control of biomass and the maintenance of cell viability in a gas-lift bioreactor. For this purpose, foam fractionation by skimming was proposed as a tool for control of continuous biomass concentration. The consequences of foaming on lignocellulosic yeast carrier losses were assessed and discussed. A steady consumption of sugars from wort, as well as consistent ethanol production, were achieved. The viability of the suspended cells in the reactor was compared with that of the cell population in the foam using flow cytometry. Results suggest that foam might be used as a promising tool to skim non-viable biomass out of the gas-lift reactor, thus ensuring the maintenance of a cell culture with optimum viability. Copyright.
机译：连续发酵是一个广为人知且经过广泛研究的过程。由于相关的高容积生产率，节省时间和较低的资本需求，因此在大量研究中都使用了固定化细胞技术（ICT）。这项工作旨在解决在啤酒发酵中大规模实施ICT的最相关障碍之一，即在气举生物反应器中控制生物量和维持细胞活力。为此，提出了通过撇取进行的泡沫分级分离作为控制连续生物质浓度的工具。评估了泡沫对木质纤维素酵母载体损失的影响并进行了讨论。麦汁中糖的消耗稳定，乙醇产量稳定。使用流式细胞术将反应器中悬浮细胞的活力与泡沫中细胞群体的活力进行比较。结果表明，泡沫可以用作从气举反应器中脱除不可行生物质的有前途的工具，从而确保维持具有最佳生存力的细胞培养物。版权。

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  • 来源
    《Journal of the Institute of Brewing》 |2014年第1期|52-59|共8页
  • 作者

    Eduardo J. Pires; Jose A. Teixeira; Tomas Branyik; Manuela Corte-Real; Antonio A. Vicente;

  • 作者单位

    IBB - Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal;

    IBB - Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal;

    Department of Biotechnology, Institute of Chemical Technology Prague, Technicka 5, 166 28, Prague 6, Czech Republic;

    Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal;

    IBB - Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    continuous fermentation; lignocellulosic yeast carrier (LCYC); excess of biomass; foam fractionation;

    机译：连续发酵木质纤维素酵母载体（LCYC）;生物量过剩泡沫分离;

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