• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of the Institute of Brewing >Acyl Homoserine Lactone Production of Brewery Process Surface Bacteria
【24h】

Acyl Homoserine Lactone Production of Brewery Process Surface Bacteria

机译：啤酒生产过程表面细菌的酰基高丝氨酸内酯生产

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to find out whether the first bacterial colonizers of brewery process surfaces after washing produce acyl homoserine lactone (AHL) quorum sensing signalling molecules. Microbial attachment and biofilm formation was studied by mounting sterile uncoated and coated stainless steel coupons onto critical places of the filling machines in three breweries. In the present study 26 previously deposited bacterial strains, as well as approximately 2,300 colonies from 76 process surface samples, were screened using reporter bacteria Chromo-bacterium violaceum CV026 and Agrobacterium tumefaciens NTLR4. AHL-producing bacteria were detected both from process samples and among the previous isolates. From the process samples, up to 15% of the screened colonies produced AHL molecules. Production of long chain AHLs was more common than short chain AHLs. The identified AHL-producing isolates belonged to the genera Pseudomonas, Serratia, Hafinia, Rah-nella, Enterobacter and Aeromonas, which all belong to commonly found primary colonizers of brewery process surfaces. The same microbial genera producing AHL molecules were found from different breweries. Brewery filling equipment is susceptible to microbial attachment and accumulation on surfaces. In the future, inhibition of quorum sensing could be one additional way of controlling biofilm formation.
机译：这项研究的目的是找出清洗后的啤酒厂加工表面的第一个细菌定殖者是否产生酰基高丝氨酸内酯（AHL）群体感应信号分子。通过将无菌的未涂覆和涂覆的不锈钢试样安装到三个啤酒厂的灌装机的关键位置，研究了微生物的附着和生物膜的形成。在本研究中，使用报告细菌紫细菌色杆菌CV026和根癌农杆菌NTLR4筛选了26个先前沉积的细菌菌株以及来自76个过程表面样品的大约2,300个菌落。从工艺样品和以前的分离株中都检测到产生AHL的细菌。从工艺样品中，多达15％的筛选菌落产生AHL分子。长链AHL的生产比短链AHL更为普遍。鉴定出的产生AHL的分离物属于假单胞菌属（Pseudomonas），沙雷氏菌属（Serratia），哈菲尼亚属（Hafinia），拉赫内拉菌（Rah-nella），肠杆菌属（Enterobacter）和气单胞菌属（Aeromonas），它们均属于啤酒加工表面的常见主要定殖者。在不同的啤酒厂中发现了相同的微生物属AHL分子。啤酒厂的灌装设备易受微生物附着和在表面堆积的影响。将来，抑制群体感应可能是控制生物膜形成的另一种方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号