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首页> 外文期刊>Journal of the Institute of Brewing >Improved Enrichment Cultivation of Beer Spoiling Lactic Acid Bacteria by Continuous Glucose Addition to the Culture
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Improved Enrichment Cultivation of Beer Spoiling Lactic Acid Bacteria by Continuous Glucose Addition to the Culture

机译：通过连续向培养物中添加葡萄糖改善啤酒变质乳酸菌的富集培养

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摘要

Lactic acid bacteria (LAB) are known as predominant beer spoilers. They cause turbidity, acidity, gas formation and off-flavors in beer by formation of side metabolites. Beer spoiling LAB have a substantial financial impact in the brewing industry making their rapid detection and identification essential. Despite the developed rapid diagnostic methods, the bottleneck in detection remains the lengthy enrichment cultivation step. This paper describes the applicability of a novel glucose auto delivery system, EnBase™, for the improved enrichment cultivation of beer spoiling LAB in MRS medium. By means of the applied system, glucose is slowly released into the culture during growth, which results in faster enrichment. Growth of Lactobacillus brevis DSM 20054T and several beer spoiling LAB was accelerated resulting in up to a 300% increase in the cell density after 48 h of cultivation compared to the commonly used MRS medium. A test of naturally contaminated beer samples indicated that the addition of glucose by means of EnBase allows faster detection of LAB in breweries.
机译：乳酸菌（LAB）被认为是主要的啤酒破坏者。它们会通过形成副代谢物而导致啤酒中的浑浊，酸度，气体形成和异味。啤酒变质乳酸菌在酿造行业中具有重大的财务影响，因此必须快速检测和鉴定。尽管开发了快速的诊断方法，但检测的瓶颈仍然是漫长的浓缩培养步骤。本文介绍了新型葡萄糖自动递送系统EnBase™在MRS培养基中改进啤酒变质LAB的富集培养的适用性。通过应用的系统，葡萄糖在生长过程中缓慢释放到培养物中，从而导致更快的富集。与常用的MRS培养基相比，培养48 h后，短乳杆菌DSM 20054T和数种啤酒变质LAB的生长加速，导致细胞密度最多增加300％。对天然污染啤酒样品的测试表明，通过EnBase添加葡萄糖可以更快地检测啤酒厂中的LAB。

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  • 来源
    《Journal of the Institute of Brewing》 |2009年第3期|177-182|共6页
  • 作者

    Sanna Taskila; Peter Neubauer; Mika Tuomola; Antje Breitenstein; Jukka Kronloef; Tomi Hillukkala;

  • 作者单位

    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P. O. Box 43100, FI-90014 Oulu, Finland;

    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P. O. Box 43100, FI-90014 Oulu, Finland Department of Biotechnology, Technische Univer-sitat Berlin, Ackerstr. 71-76, D-13355 Berlin, Germany;

    Thermo Fisher Scientific Ltd., Ratastie 2, P. O. Box 100, FI-01621, Vantaa, Finland;

    Scanbec GmbH. Weinbergweg 23, D-03120 Halle/S., Germany;

    Hartwall Ltd., Kasaajankatu 13, P. O. Box 44, FI-15101 Lahti, Finland;

    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P. O. Box 43100, FI-90014 Oulu, Finland;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    beer spoilage; contaminant detection; enrichment cultivation; lactic acid bacteria; lactobacillus;

    机译：啤酒变质;污染物检测;浓缩栽培;乳酸菌乳杆菌;

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