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首页> 外文期刊>Journal of the Institute of Brewing >Removal of Microbial Biofilms from Dispense Equipment: The Effect of Enzymatic Pre-digestion and Detergent Treatment
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Removal of Microbial Biofilms from Dispense Equipment: The Effect of Enzymatic Pre-digestion and Detergent Treatment

机译：从分配设备去除微生物生物膜：酶预消化和洗涤剂处理的影响

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摘要

Microbial biofilms can form in dispense outlets as a result of poor or inadequate cleaning and can be difficult to remove using conventional practices. Enzymatic cleaners might help to remove biofilms by degrading the exopolysaccharide layers in which the microbes are embedded. A multispecies biofilm comprising wild type dispense isolates of Flavimonas oryzihabitans, Lactobacillus brevis, Leuconostoc mesenteroides and Saccharomyces cerevisiae was generated on a section of tubing and fitted into a pilot dispense system, which was left uncleaned for 12 weeks. After cleaning approximately 10~4 viable aerobes and 10~3 viable anaerobes were still present. Stainless steel coupons and pieces of dispense line contaminated with biofilm were incubated in the laboratory with an enzyme mix containing varying proportions of α-amylase, β-glucuronidase, glucose oxidase, dextranase, protease and pectinase. Cultures grown on stainless steel had significantly (F pr. < 0.05) less viable cells than nonenzyme treated biofilms, but this was dependent on the microbial species. Typically Lactobacillus brevis was most susceptible to the enzyme treatment. Cultures grown on dispense line were much more resistant to enzymatic digestion. Pre-digestion with protease was most effective for removal of Lactobacillus brevis and Leuconostoc mesenteroides but not for Saccharomyces cerevisiae and Flavimonas oryzihabitans. In the simulated bar, predigestion with protease reduced the viable cell count by 0.64 log units for the aerobes and 1.9 log units for the anaerobes. This study demonstrates that pre-digestion with enzyme solutions before line cleaning is useful for treating heavily contaminated lines in trade.
机译：由于清洁不良或不充分，可能会在分配出口形成微生物膜，并且使用传统方法可能难以去除。酶清洁剂可能会通过降解嵌入微生物的胞外多糖层来帮助去除生物膜。在一段油管上产生了包含野生型黄精黄藻，短乳杆菌，短肠乳突性肠杆菌和酿酒酵母的野生型分配分离菌的多物种生物膜，并将其安装在未分配清洁的试验分配系统中，该系统无需清洗12周。清洗后，仍然存在大约10〜4个活菌和10〜3个活菌。在实验室中，将被生物膜污染的不锈钢试样和分配管线与含有不同比例的α-淀粉酶，β-葡糖醛酸糖苷酶，葡萄糖氧化酶，葡聚糖酶，蛋白酶和果胶酶的酶混合物一起孵育。与未经酶处理的生物膜相比，在不锈钢上生长的培养物的活细胞明显少（F pr。<0.05），但这取决于微生物种类。通常，短乳杆菌对酶处理最敏感。在分配线上生长的培养物更耐酶消化。用蛋白酶预消化最有效的方法是去除短乳杆菌和间肠乳突球菌，但对酿酒酵母和米黄霉菌不是最有效。在模拟条中，用蛋白酶进行的预消化将需氧菌的活细胞数减少了0.64 log单位，将厌氧菌的生存细胞数减少了1.9 log单位。这项研究表明，在清洗生产线之前用酶溶液预先消化可用于处理行业中严重污染的生产线。

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