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首页> 外文期刊>Journal of the Institute of Brewing >Regio- and Stereoselectivity of Malt Lipoxygenases LOX1 and LOX2
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Regio- and Stereoselectivity of Malt Lipoxygenases LOX1 and LOX2

机译：麦芽脂加氧酶LOX1和LOX2的区域和立体选择性。

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摘要

The characterisation of lipoxygenases LOX1 and LOX2 and hydroperoxyoctadecadienoic acid (HPODE) degrading enzymes from barley green malt is reported. Hydroxylapatite chromatography (HAC) and isoelectric focussing (IEF) were performed to separate and purify LOX isoenzymes. The regio- and stereo-selectivity of LOX1 and LOX2 towards linoleic acid as substrate was characterised. HAC purified isoenzyme LOX1 showed a 9-HPODE:13-HPODE ratio of 75:25 and LOX2 a ratio of 39:61. IEF separated LOX1 and LOX2 transformed linoleic acid to 9-:13-HPODE ratios of 90:10, and 13:87, respectively. 9-HPODE stereoisomers from LOX1 exhibited a S:R ratio of 93:7 and 13-HPODE from LOX2 a S:R ratio of 89:11. However, the minor regioisomers were analysed with S:R = 48:52 (LOX1, 13-HPODE) and 40:60 (LOX2, 9-HPODE). These results indicate a complete LOX isoenzyme separation by IEF. Hydro-peroxide-metabolising enzymes, which were investigated in the IEF fractions, did not interfere with the dual position specificities of LOX isoenzymes.
机译：据报道，来自大麦绿麦芽的脂氧合酶LOX1和LOX2以及氢过氧十八碳二烯酸（HPODE）降解酶的表征。进行了羟基磷灰石色谱（HAC）和等电聚焦（IEF）分离和纯化LOX同工酶。表征了LOX1和LOX2对亚油酸作为底物的区域和立体选择性。 HAC纯化的同工酶LOX1的9-HPODE：13-HPODE比例为75：25，LOX2的比例为39:61。 IEF将LOX1和LOX2转化的亚油酸分离为9 :: 13-HPODE的比例分别为90:10和13:87。 LOX1的9-HPODE立体异构体的S：R比为93：7，LOX2的13-HPODE的S：R比为89:11。但是，次要区域异构体的分析条件为S：R = 48:52（LOX1，13-HPODE）和40:60（LOX2，9-HPODE）。这些结果表明，通过IEF可以完全分离LOX同工酶。在IEF馏分中研究的过氧化氢代谢酶不会干扰LOX同工酶的双重位置特异性。

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