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首页> 外文期刊>Journal of the Institute of Brewing >Pb~(2+) Inhibits Competitively Flocculation of Saccharomyces cerevisiae
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Pb~(2+) Inhibits Competitively Flocculation of Saccharomyces cerevisiae

机译：Pb〜（2+）竞争抑制酿酒酵母的絮凝

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摘要

Pb~(2+) inhibited calcium-induced flocculation in Flo1 (S646-1B) and NewFlo phenotype strains (NCYC 1190, NCYC 1195 and NCYC 1364) of Saccharomyces cerevisiae. Flocculation was restored after washing with water or EDTA, which suggests a reversible binding of Pb~(2+) to yeast cell walls. Pb~(2+) probably inhibited flocculation by competing with Ca~(2+), since Pb~(2+) inhibition was alleviated by excess of Ca~(2+). Using a fluorescent avidin-fluorescein isothiocyanate (Avidin-FITC) probe, active cell surface flocculation lectins, in the presence of Ca~(2+) ions, were visualized. Conversely, Avidin-FITC was not fixed to yeast walls of flocculent cells, in the presence of Pb~(2+) ions or in the simultaneous presence of 0.05 mM Ca~(2+) and 0.4 mM Pb~(2+). These results suggest that Pb~(2+) ions were not able to induce the correct conformation of the lectin-like component and reinforces the hypothesis that Pb~(2+) ions compete for the same "calcium site" of flocculation zymolectins.
机译：Pb〜（2+）抑制了酿酒酵母Flo1（S646-1B）和NewFlo表型菌株（NCYC 1190，NCYC 1195和NCYC 1364）中钙诱导的絮凝。用水或EDTA洗涤后絮凝得以恢复，这表明Pb〜（2+）与酵母细胞壁可逆结合。由于过量的Ca〜（2+）减轻了Pb〜（2+）的抑制作用，因此Pb〜（2+）可能通过与Ca〜（2+）竞争而抑制了絮凝。使用荧光亲和素-异硫氰酸荧光素（Avidin-FITC）探针，在存在Ca〜（2+）离子的情况下，可以看到活性细胞表面絮凝凝集素。相反，在存在Pb〜（2+）离子或同时存在0.05 mM Ca〜（2+）和0.4 mM Pb〜（2+）的情况下，抗生物素蛋白-FITC不能固定在絮状细胞的酵母壁上。这些结果表明，Pb〜（2+）离子不能诱导凝集素样成分的正确构象，并加强了Pb〜（2+）离子竞争絮凝酶连接素的相同“钙位点”的假设。

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