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首页> 外文期刊>Journal of Liquid Chromatography & Related Technologies >HIGHLY SENSITIVE LC-MS/MS METHOD FOR THE DETERMINATION OF CLOZAPINE IN RAT PLASMA: APPLICATION TO A PRECLINICAL PHARMACOKINETIC STUDY
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HIGHLY SENSITIVE LC-MS/MS METHOD FOR THE DETERMINATION OF CLOZAPINE IN RAT PLASMA: APPLICATION TO A PRECLINICAL PHARMACOKINETIC STUDY

机译：高灵敏度LC-MS / MS法测定大鼠血浆中的氯氮平：在临床药动学研究中的应用

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A highly sensitive, rapid assay method has been developed and validated for the quantification of clozapine in rat plasma using LC-MS/MS. The extraction process involved plasma protein precipitation with acetonitrile and elution with a mobile phase constituted by acetonitrile and acidic water (80/20), at 0.8 mL/min flow rate (split 1:3). Chromatographic separation was performed by C18 Symmetry column (4.6 × 75 mm, 3.5 µm). The lower limit of quantification was 1 ng/mL and the linearity was observed between 1-1000 ng/mL with a determination coefficient 0.9810. The intra- and inter-day precisions were less or equal to 8.38 and 4.36%, respectively. The method showed sensitivity, linearity, precision, accuracy, and specificity necessary for quantification of plasma clozapine in preclinical pharmacokinetics studies.View full textDownload full textKeywordsclozapine, LC-MS/MS, pharmacokinetics, preclinical studies, quinine, rat plasmaRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10826076.2011.639118
机译：已经开发出一种高度灵敏，快速的测定方法，并已使用LC-MS / MS验证了氯氮平在大鼠血浆中的定量作用。提取过程涉及血浆蛋白用乙腈沉淀，并用由乙腈和酸性水（80/20）组成的流动相洗脱，流速为0.8 mL / min（按1：3）。色谱分离是由C18对称性列（4.6×75毫米，3.5 µm）。定量的下限为1 ng / mL，线性在1-1000 ng / mL之间，测定系数> 0.9810。日内和日间精度分别小于或等于8.38和4.36％。该方法显示了在临床前药代动力学研究中定量测定氯氮平所需的灵敏度，线性，精密度，准确度和特异性。查看全文下载全文：“ Taylor＆Francis Online”，services_compact：“ citeulike，netvibes，twitter，technorati，delicious，linkedin，facebook，stumbleupon，digg，google，more”，pubid：“ ra-4dff56cd6bb1830b”};添加到候选列表链接永久链接http://dx.doi.org/10.1080/10826076.2011.639118

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