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首页> 外文期刊>Journal of Materials Research >Effect of sterilization processes on nanostructuredTi6AI4V surfaces obtained by electropolishing
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Effect of sterilization processes on nanostructuredTi6AI4V surfaces obtained by electropolishing

机译：灭菌方法对电解抛光获得的纳米结构u6AI4V表面的影响

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Electrochemical treatments, such as electropolishing on titanium alloys, are used to promote the formation of nanostructured surfaces, which can contribute to the bone regeneration process. However, sterilization methods can change the superficial and physicochemical properties of the biomaterials. The objective of this work was to evaluate the effect of three sterilization methods (air plasma, ethanol + PBS, and autoclave sterilizations) on nanostructured Ti6Al4V surfaces properties obtained by electrochemical treatment. These methods, especially the first two, have been widely used in literature, yet few studies in the literature highlight the changes on the surface of the samples. The nanostructures were obtained by electropolishing in a H2SO4/HF/glycerine solution, at 25 V and at 7 degrees C for 4 min. Samples were characterized by atomic force microscopy (AFM), profilometry, and wettability. Samples were seeded with hESC-MPs, and the cell number was measured. The air plasma sterilization did not promote changes in nanometric morphology and roughness of the Ti6Al4V nanostructured samples. Unlike air plasma sterilization, the ethanol + PBS and the autoclave sterilizations, which strongly affected the nanostructured surface morphology and properties, and, consequently, the cellular viability after 7 days of contact with human embryonic stem cell-derived mesenchymal progenitors (hESC-MPs).
机译：电化学处理，例如在钛合金上电抛光，用于促进纳米结构表面的形成，这可以有助于骨再生过程。然而，灭菌方法可以改变生物材料的浅表和物理化学性质。这项工作的目的是评估三种灭菌方法（空气血浆，乙醇+ PBS和高压釜灭菌）对通过电化学处理获得的纳米结构的Ti6Al4V表面的影响。这些方法，尤其是前两个，已广泛用于文学中，但文献中的几个研究突出了样品表面的变化。通过在H 2 SO 4 / HF /甘油溶液中在25V和7℃下电挖掘获得纳米结构4分钟。样品以原子力显微镜（AFM），轮廓测定法和润湿性为特征。用HESC-MPS接种样品，测量细胞数。空气等离子体灭菌并未促进Ti6Al4V纳米结构样品的纳米形态和粗糙度的变化。与空气等离子体灭菌不同，乙醇+ PBS和高压釜灭菌，其强烈影响纳米结构的表面形态和性质，因此，与人胚胎干细胞的间充质祖细胞（HESC-MPS）接触7天后的细胞活力。

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