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首页> 外文期刊>Journal of Materials Research >Single-pot biofabrication of living fibers for tissue engineering applications
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Single-pot biofabrication of living fibers for tissue engineering applications

机译：用于组织工程应用的活性纤维的单锅生物制造

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摘要

In this work, morphology, viability, and metabolism of the amniotic mesenchymal stem cells conditioned with different citric acid (CA)/media ratios were investigated using rhodamine-phalloidin/4,6-diamidino-2-phenylindole staining, live/dead assay, proliferating cell nuclear antigen, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL assay). The cells cultured in 25:75 CA/media displayed well spread actin filaments with a prominent nucleus and evidenced optimum viability. The gelation kinetics of chitosan solution in CA/media (25:75) was monitored via dynamic time sweep analysis on a rheometer. The chemical cross-linking of chitosan with CA was confirmed by nuclear magnetic resonance studies. Subsequently, chitosan solution was extruded in CA/media bath containing cells under benign conditions to form cell-laden fibers (living fibers). The prelabeled cells imaged immediately after fiber formation confirmed the attachment of the cells on the fibers. This approach has several advantages including instantaneous gelation, tunable mechanical properties, and adjustable biodegradability that can provide a platform technology for creating viable three dimensional (3D) building blocks for tissue engineering applications.
机译：在这项工作中，使用若丹明-鬼笔环肽/ 4,6-二mid基-2-苯基吲哚染色，活体/死分析，研究了以不同柠檬酸（CA）/培养基比率调节的羊膜间充质干细胞的形态，活力和代谢增殖细胞核抗原和末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL分析）。在25:75 CA /培养基中培养的细胞显示出分布良好的肌动蛋白丝，具有突出的细胞核，并具有最佳的生存能力。通过流变仪上的动态时间扫描分析来监测壳聚糖溶液在CA /介质（25:75）中的凝胶动力学。核磁共振研究证实了壳聚糖与CA的化学交联。随后，将壳聚糖溶液在良性条件下在含有细胞的CA /培养基浴中挤出以形成载有细胞的纤维（活性纤维）。在纤维形成后立即成像的预标记细胞证实了细胞在纤维上的附着。这种方法具有多个优点，包括瞬时胶凝，可调节的机械性能和可调节的生物降解性，可为创建用于组织工程应用的可行的三维（3D）构建基块提供平台技术。

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  • 来源
    《Journal of Materials Research》 |2018年第14期|2019-2028|共10页
  • 作者

    Ghosh Paulomi; Rameshbabu Arun Prabhu; Das Dipankar; Subramanian Bhuvaneshwaran; Samanta Sintu Kumar; Roy Sabyasachi; Pal Sagar; Ghosh Sudip Kumar; Dhara Santanu;

  • 作者单位

    Indian Inst Technol Kharagpur, Sch Med Sci & Technol, Biomat & Tissue Engn Lab, Kharagpur 721302, W Bengal, India;

    Indian Inst Technol Kharagpur, Sch Med Sci & Technol, Biomat & Tissue Engn Lab, Kharagpur 721302, W Bengal, India;

    Indian Sch Mines, Dept Appl Chem, Polymer Chem Lab, Dhanbad 826004, India;

    Indian Inst Technol Kharagpur, Sch Med Sci & Technol, Biomat & Tissue Engn Lab, Kharagpur 721302, W Bengal, India;

    Indian Inst Technol Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India;

    Midnapore Med Coll, Dept Gynaecol, Paschim Medinipur 721101, India;

    Indian Sch Mines, Dept Appl Chem, Polymer Chem Lab, Dhanbad 826004, India;

    Indian Sch Mines, Dept Appl Chem, Polymer Chem Lab, Dhanbad 826004, India;

    Indian Inst Technol Kharagpur, Sch Med Sci & Technol, Biomat & Tissue Engn Lab, Kharagpur 721302, W Bengal, India;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biomaterial; polymer;

    机译：生物材料;聚合物;

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