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首页> 外文期刊>Journal of Materials Research >Spatial localization of Mms6 during biomineralization of Fe_3O_4 nanocrystals in Magnetospirillum magneticum AMB-1
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Spatial localization of Mms6 during biomineralization of Fe_3O_4 nanocrystals in Magnetospirillum magneticum AMB-1

机译：磁性螺旋藻AMB-1中Fe_3O_4纳米晶体生物矿化过程中Mms6的空间定位。

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摘要

Magnetotactic bacteria mineralize nanometer-size crystals of magnetite (Fe_3O_4) through a series of protein-mediated reactions that occur inside of organelles called magnetosomes. Mms6 is a transmembrane protein thought to play a key role in magnetite mineralization. We used both electron and fluorescent microscopy to examine the subcellular location of Mms6 protein within single cells of Magnetospirillum magneticum AMB-1 using Mms6-specific antibodies. We also purified magnetosomes from M. magneticum to determine if Mms6 was physically attached to magnetite crystals. Our results show that Mms6 proteins are present during crystal growth, and Mms6 is found in direct contact with the magnetite crystals or within the lipid/protein membrane surrounding the magnetite crystals. Mms6 was not detected at other subcellular locations within the bacteria or isolated fractions. Because Mms6 was found to completely surround the magnetosomes rather than being localized to one specific area of the magnetosome, it appears that this protein could act on the entire magnetite crystal during the biomineralization process. This supports a model in which Mms6 functions to regulate Fe_3O_4 crystal morphology. This knowledge is important for future in vitro experiments utilizing Mms6 to synthesize tailored nanomagnets with specific physical or magnetic properties.
机译：趋磁细菌通过一系列称为细胞小体的细胞器内部发生的蛋白质介导的反应，使纳米级磁铁矿（Fe_3O_4）晶体矿化。 Mms6是一种跨膜蛋白，被认为在磁铁矿矿化中起关键作用。我们使用电子显微镜和荧光显微镜，使用Mms6特异性抗体检查Mms6蛋白在Magnetospirillum magneticum AMB-1单细胞内的亚细胞位置。我们还从M. magneticum纯化了磁小体，以确定Mms6是否物理附着到磁铁矿晶体上。我们的结果表明，Mms6蛋白在晶体生长过程中存在，并且Mms6被发现与磁铁矿晶体直接接触或围绕磁铁矿晶体的脂质/蛋白质膜内。在细菌或分离的部分中的其他亚细胞位置未检测到Mms6。因为发现Mms6完全围绕着磁小体，而不是局限于磁小体的一个特定区域，所以看来这种蛋白质可以在生物矿化过程中作用于整个磁铁矿晶体。这支持其中Mms6用于调节Fe_3O_4晶体形态的模型。该知识对于将来利用Mms6合成具有特定物理或磁性特性的定制纳米磁体的体外实验非常重要。

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  • 来源
    《Journal of Materials Research》 |2016年第5期|527-535|共9页
  • 作者

    Zachery Oestreicher; Eric Mumper; Carol Gassman; Dennis A. Bazylinski; Steven K. Lower; Brian H. Lower;

  • 作者单位

    School of Natural System, College of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan,School of Environment and Natural Resources, The Ohio State University, Columbus, Ohio 43210, USA;

    School of Earth Sciences, The Ohio State University, Columbus, Ohio 43210, USA;

    Department of Chemistry, Columbia Basin College, Pasco, Washington 99301, USA;

    School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, Nevada 89154, USA;

    School of Earth Sciences, The Ohio State University, Columbus, Ohio 43210, USA,School of Environment and Natural Resources, The Ohio State University, Columbus, Ohio 43210, USA;

    School of Environment and Natural Resources, The Ohio State University, Columbus, Ohio 43210, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
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