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Tunable glass reference materials for quantitative backscattered electron imaging of mineralized tissues

机译:可调谐玻璃参考材料,用于矿化组织的定量背散射电子成像

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摘要

Backscattered electron microscopy provides gray-level contrast resulting from variations in atomic composition. Through the use of reference materials, quantitative backscattered electron (qBSE) imaging can be used to measure the mineral content of mineralized tissues at submicron resolution. We have developed novel tunable reference materials that can be adjusted for analysis of an individual tissue or a wide range of tissues with variable atomic density. As an alternative to conventional metallic reference materials, these amorphous materials maintain long-term stability and possess no long-range order that may induce channeling contrast. Using these reference materials, we characterized the mineral content of a broad range of mineralized tissues from immature mouse femur to whale bulla. Mineral volume fraction correlated to more traditional measurements of mineral content with microcomputed tomography and ashing techniques. Further, we demonstrate the advantage of location-matched measurements of nanomechanical properties and qBSE mineral content.
机译:反向散射电子显微镜可提供由于原子组成变化而产生的灰度对比度。通过使用参考材料,定量背散射电子(qBSE)成像可用于以亚微米分辨率测量矿化组织的矿物质含量。我们已经开发出新颖的可调参考材料,可以对其进行调整,以分析单个组织或具有可变原子密度的各种组织。作为常规金属参比材料的替代品,这些无定形材料保持长期稳定性,并且不具有可能引起通道对比的远距离有序。使用这些参考材料,我们表征了从未成熟的小鼠股骨到大鲸鱼的各种矿化组织的矿物质含量。矿物体积分数与使用微计算机断层扫描和灰化技术进行的更传统的矿物含量测量相关。此外,我们证明了纳米机械性能和qBSE矿物含量的位置匹配测量的优势。

著录项

  • 来源
    《Journal of Materials Research》 |2012年第19期|p.2568-2577|共10页
  • 作者单位

    Department of Mechanical Engineering, University of Colorado, Boulder, Colorado 80309 Materials Reliability Division, NIST, Boulder, Colorado 80305;

    Materials Reliability Division, NIST, Boulder, Colorado 80305;

    Physics Department, Coe College, Cedar Rapids, Iowa 52402;

    Department of Mechanical Engineering, University of Colorado, Boulder, Colorado 80309;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
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