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首页> 外文期刊>Journal of Materials Research >Comparative and quantitative investigation of cell labeling of a 12-nm DMSA-coated Fe_3O_4 magnetic nanoparticle with multiple mammalian cell lines
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Comparative and quantitative investigation of cell labeling of a 12-nm DMSA-coated Fe_3O_4 magnetic nanoparticle with multiple mammalian cell lines

机译：比较和定量研究具有多个哺乳动物细胞系的12nm DMSA涂层Fe_3O_4磁性纳米粒子的细胞标记

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摘要

This work investigated the cell labeling of 12-nm meso-2,-3-dimercaptosuccinic acid (DMSA)-coated Fe_3O_4 magnetic nanoparticles with multiple mammalian cells. Six different cells, including RAW264.7, Hepal-6, THP-1, HepG2, HeLa, and HL-7702, were treated with the nanoparticles at various concentrations (20~100 μg/mL) for different times (2~72 h), and the labeling effect was evaluated by observing the intracellular internalization of the nanoparticles with Prussian blue staining and measuring the corresponding cellular iron loading with colorimetric assay. The results demonstrated that the nanoparticles could label all cells studied. However, the labeling efficiency was not the same between different cells, which depended on the cell types, the nanoparticles' concentration, and the time of treating cells with the nanoparticles. In comparison, RAW264.7 was labeled more effectively than other cells at any concentration of the nanoparticles. The iron loading of RAW264.7 significantly increased with the concentration of the nanoparticles and the treatment time. However, both human liver cells (HepG2 and HL-7720) were labeled with the lowest iron loading. The measurement of cell viability revealed that the growth of all cells was not affected by the nanoparticles at a common in vivo application dose of iron nanoparticles (30 |Jg/mL), demonstrating that the nanoparticles have better biocompatability.
机译：这项工作研究了具有多个哺乳动物细胞的12-nm meso-2，-3-二巯基琥珀酸（DMSA）涂层的Fe_3O_4磁性纳米颗粒的细胞标记。用不同浓度（20〜100μg/ mL）的纳米颗粒处理RAW264.7，Hepal-6，THP-1，HepG2，HeLa和HL-7702等六个不同的细胞不同的时间（2〜72 h） ），并通过普鲁士蓝染色观察纳米颗粒的细胞内内化并用比色法测量相应的细胞铁载量来评估标记效果。结果表明，纳米颗粒可以标记所有研究的细胞。但是，标记效率在不同细胞之间并不相同，这取决于细胞类型，纳米颗粒的浓度以及用纳米颗粒处理细胞的时间。相比之下，在任何浓度的纳米颗粒上，RAW264.7的标记都比其他细胞更有效。 RAW264.7的铁含量随纳米粒子的浓度和处理时间而显着增加。但是，两种人类肝细胞（HepG2和HL-7720）都标记了最低的铁含量。细胞活力的测量结果表明，在铁纳米颗粒的常规体内应用剂量（30μg/ mL）下，所有细胞的生长不受纳米颗粒的影响，表明纳米颗粒具有更好的生物相容性。

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  • 来源
    《Journal of Materials Research》 |2011年第6期|p.822-831|共10页
  • 作者

    Yingxun Liu; Jinke Wang;

  • 作者单位

    State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering,Southeast University, Nanjing 210096, China;

    State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering,Southeast University, Nanjing 210096, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
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