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首页> 外文期刊>Journal of Materials Research >Cell Labeling Efficiency Of Layer-by-layer Self-assembly Modified Silica Nanoparticles
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Cell Labeling Efficiency Of Layer-by-layer Self-assembly Modified Silica Nanoparticles

机译：层状自组装改性二氧化硅纳米粒子的细胞标记效率

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摘要

In the present study, we compared cytotoxicity and cell uptake of silica nanoparticles with four different surface coatings generated through layer-by-layer self-assembly. Rabbit mesenchymal stem cells (rMSCs) were labeled with silica nanoparticles of different coatings including poly(ethyleneimine) (PEI), poly (allylamine hydrochloride) (PAH), poly(anetholesulfonic acid, sodium salt) (PAS), and dextran sulfate. The MTT [3-(4, 5-dimethylthiazol-2)-2, 5-diphenyl-2H-tetrazolium bromide] test was performed to quantify the cell biocompatibility. The cellular uptake of those silica nanoparticles was determined by flow cytometry and confocal laser scanning microscopy. The results showed that all examined silica nanoparticles were stable in aqueous phase with high monodispersity. Labeled rMSCs are unaffected in their viability, apoptosis, and differentiation capacities. The silica nanoparticle-coated synthetic polycations such as PEI or PAH have higher cell internalization than negatively charged polyelectrolytes. The ability to control cell uptake of different particles may have applications in cell labeling, cell separation, and other biomedical applications.
机译：在本研究中，我们比较了通过逐层自组装产生的四种不同表面涂层对二氧化硅纳米颗粒的细胞毒性和细胞摄取。兔间充质干细胞（rMSCs）用不同涂层的二氧化硅纳米颗粒进行标记，这些涂层包括聚（乙烯亚胺）（PEI），聚（烯丙胺盐酸盐）（PAH），聚（邻苯二甲磺酸，钠盐）（PAS）和硫酸葡聚糖。进行MTT [3-（4，5-二甲基噻唑-2）-2，5-二苯基-2H-溴化四唑]测试以量化细胞的生物相容性。通过流式细胞术和共聚焦激光扫描显微镜确定那些二氧化硅纳米颗粒的细胞摄取。结果表明，所有检测的二氧化硅纳米粒子在水相中均具有高单分散性，并且是稳定的。标记的rMSCs的生存力，凋亡和分化能力均不受影响。二氧化硅纳米颗粒涂覆的合成聚阳离子（例如PEI或PAH）比带负电的聚电解质具有更高的电池内化度。控制不同颗粒的细胞摄取的能力可能在细胞标记，细胞分离和其他生物医学应用中具有应用。

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