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首页> 外文期刊>Journal of Materials Research >Formation of composite gel fiber from cellulose acetate and zirconium tetra-n-butoxide and entrap-immobilization of β-galactosidase on the fiber
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Formation of composite gel fiber from cellulose acetate and zirconium tetra-n-butoxide and entrap-immobilization of β-galactosidase on the fiber

机译：由醋酸纤维素和四正丁酸锆形成复合凝胶纤维并将β-半乳糖苷酶包埋固定在纤维上

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摘要

The formation of a composite gel fiber from cellulose acetate and zirconium tetra-n-butoxide was examined. The gel was thought to have formed by the coordination of OH and CO groups of cellulose to zirconium, taking six coordination numbers around the zirconium. The gel fiber had good stability in common solvents, phosphate solution, and electrolyte solution. Then, under mild conditions, a β-galactosidase was entrap-immobilized on it. The apparent Michaelis constant of the immobilized β-galactosidase was larger than that of native β-galactosidase, whereas the opposite tendency was observed for the maximum reaction velocity. The activity of the immobilized β-galactosidase increased as the fiber diameter decreased. These findings indicated that lactose hydrolysis took place in the vicinity of the fiber surface. The immobilized β-galactosidase had a higher thermal stability than the native type.
机译：研究了由乙酸纤维素和四正丁酸锆形成的复合凝胶纤维。据认为该凝胶是由纤维素的OH和CO基团与锆的配位形成的，在锆周围具有六个配位数。凝胶纤维在普通溶剂，磷酸盐溶液和电解质溶液中具有良好的稳定性。然后，在温和的条件下，将β-半乳糖苷酶诱集固定在其上。固定的β-半乳糖苷酶的表观米氏常数大于天然β-半乳糖苷酶，而在最大反应速度上观察到相反的趋势。固定的β-半乳糖苷酶的活性随纤维直径的减小而增加。这些发现表明乳糖水解发生在纤维表面附近。固定的β-半乳糖苷酶具有比天然类型更高的热稳定性。

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  • 来源
    《Journal of Materials Research》 |2003年第3期|p.672-676|共5页
  • 作者

    K. Nakane; T. Ogihara; N. Ogata; Y. Kurokawa;

  • 作者单位

    Department of Materials Science and Engineering, Faculty of Engineering, Fukui University, Bunkyo 3-9-1, Fukui 910-8507, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 工程材料学;
  • 关键词

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