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首页> 外文期刊>Journal of materials science >Reseeding endothelial cells with fibroblasts to improve the re-endothelialization of pancreatic acellular scaffolds
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Reseeding endothelial cells with fibroblasts to improve the re-endothelialization of pancreatic acellular scaffolds

机译：用成纤维细胞重播内皮细胞，以改善胰腺脱细胞支架的再内皮化

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摘要

Pancreatic transplantation remains the only cure for diabetes, but the shortage of donors limits its clinical application. Whole organ decellularized scaffolds offer a new opportunity for pancreatic organ regeneration; however inadequate endothelialization and vascularization can prevent sufficient transport of oxygen and nutrient supplies to the transplanted organ, as well as leading unwanted thrombotic events. In the present study, we explored the re-endothelialization of rat pancreatic acellular scaffolds via circulation perfusion using human skin fibroblasts (FBs) and human umbilical vein endothelial cells (HUVECs). Our results revealed that the cell adhesion rate when these cells were co-cultured was higher than under control conditions, and this increase was associated with increased release of growth factors including VEGF, FGFb, EGF, and IGF-1 as measured by ELISA. When these recellularized organs were implanted in vivo for 28 days in rat dorsal subcutaneous pockets, we found that de novo vasculature formation in the co-culture samples was superior to the control samples. Together these results suggest that endothelial cell and FB co-culture enhances the re-endothelialization and vascularization of pancreatic acellular scaffolds.[GRAPHICS].
机译：胰腺移植仍然是治疗糖尿病的唯一方法，但是捐赠者的短缺限制了其临床应用。全器官脱细胞支架为胰腺器官再生提供了新的机会。然而，内皮化和血管化不足会阻止氧气和营养物质向移植器官的充分运输，并导致不必要的血栓形成事件。在本研究中，我们探索了使用人皮肤成纤维细胞（FBs）和人脐静脉内皮细胞（HUVECs）通过循环灌注对大鼠胰腺脱细胞支架的重新内皮化作用。我们的结果表明，这些细胞共培养时的细胞粘附率高于对照条件，并且这种增加与通过ELISA测定的包括VEGF，FGFb，EGF和IGF-1在内的生长因子释放增加有关。当这些重新细胞化的器官在大鼠背侧皮下口袋中体内植入28天后，我们发现共培养样品中的新生血管形成优于对照样品。这些结果共同表明，内皮细胞和FB共培养可增强胰腺脱细胞支架的再内皮化和血管化。[GRAPHICS]。

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  • 来源
    《Journal of materials science》 |2019年第7期|85.1-85.12|共12页
  • 作者

    Xu Liancheng; Huang Yan; Wang Dongzhi; Zhu Shajun; Wang Zhiwei; Yang Yumin; Guo Yibing;

  • 作者单位

    Nantong Univ Dept Gen Surg Affiliated Hosp Nantong Peoples R China|Nantong Univ Res Ctr Clin Med Affiliated Hosp Nantong Peoples R China;

    Nantong Univ Res Ctr Clin Med Affiliated Hosp Nantong Peoples R China;

    Nantong Univ Dept Gen Surg Affiliated Hosp Nantong Peoples R China;

    Nantong Univ Key Lab Neuroregenerat Neural Regenerat Coinnovat Ctr Jiangsu Prov Nantong Peoples R China;

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