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首页> 外文期刊>Journal of materials science >A tooth-binding antimicrobial peptide to prevent the formation of dental biofilm
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A tooth-binding antimicrobial peptide to prevent the formation of dental biofilm

机译：结合牙齿的抗菌肽可防止牙齿生物膜的形成

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Dental caries is primarily caused by pathogenic bacteria infection, and Streptococcus mutans is considered a major cariogenic pathogen. Moreover, antimicrobial peptides have been considered an alternative to traditional antibiotics in treating caries. This study aimed to design a tooth-binding antimicrobial peptide and evaluate its antimicrobial efficacy against S. mutans. An antimicrobial peptide of polyphemusin I (PI) was modified by grafting a tooth-binding domain of diphosphoserine (Ser(p)-Ser(p)-) to create the peptide of Ser(p)-Ser(p)-polyphemusin I (DPS-PI). PI and DPS-PI were synthesized by Fmoc solid-phase peptide synthesis. The minimum inhibitory concentration of PI and DPS-PI against S. mutans were tested. Scanning electron microscopy (SEM) were used to observe the growth of S. mutans on PI and DPS-PI treated enamel surfaces. The growth of S. mutans was evaluated by optical density (OD) at 590nm. Inhibition of dental plaque biofilm development in vivo were investigated. The cytocompatibility to bone mesenchymal stem cells (BMSCs) was tested. The MIC of PI and DPS-PI were 40 and 80g/ml, respectively. SEM images showed that S. mutans were sparsely distributed on the DPS-PI treated enamel surface. OD findings indicated that DPS-PI maintained its inhibition effect on S. mutans growth after 24h. The incisor surfaces of rabbits treated with DPS-PI developed significantly less dental plaque biofilm than that on PI treated surfaces. The DPS-PI had good biocompatibility with the cells. We successfully constructed a novel tooth-binding antimicrobial peptide against S. mutans in vitro and inhibited dental plaque biofilm development in vivo. DPS-PI may provide a feasible alternative to conventional antibiotics for the prevention and treatment of dental caries.[GRAPHICS].
机译：龋齿主要是由致病菌感染引起的，而变形链球菌被认为是主要的致龋性病原体。此外，在治疗龋齿方面，抗菌肽被认为是传统抗生素的替代品。这项研究旨在设计一种结合牙齿的抗菌肽，并评估其对变形链球菌的抗菌功效。通过接枝二磷酸丝氨酸（Ser（p）-Ser（p）-）的牙齿结合域来修饰多phemusin I（PI）的抗菌肽，以创建Ser（p）-Ser（p）-polyphemusin I的肽（ DPS-PI）。 PI和DPS-PI通过Fmoc固相肽合成法合成。测试了PI和DPS-PI对变形链球菌的最小抑制浓度。使用扫描电子显微镜（SEM）观察变形链球菌在PI和DPS-PI处理的牙釉质表面上的生长。通过590nm处的光密度（OD）评估变形链球菌的生长。研究了在体内抑制牙菌斑生物膜的发展。测试了与骨髓间充质干细胞（BMSCs）的细胞相容性。 PI和DPS-PI的MIC分别为40和80g / ml。 SEM图像显示变形链球菌稀疏分布在DPS-PI处理的牙釉质表面上。 OD结果表明DPS-PI在24小时后仍保持其对变形链球菌生长的抑制作用。用DPS-PI处理的兔子的切牙表面形成的牙菌斑生物膜明显少于PI处理的表面。 DPS-PI与细胞具有良好的生物相容性。我们在体外成功构建了一种新型的牙齿变形链球菌抗生抗菌肽，并在体内抑制了牙菌斑生物膜的发育。 DPS-PI可能为预防和治疗龋齿提供一种替代常规抗生素的可行替代方法。[GRAPHICS]。

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