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首页> 外文期刊>Journal of materials science >Biomechanical and structural changes following the decellularization of bovine pericardial tissues for use as a tissue engineering scaffold
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Biomechanical and structural changes following the decellularization of bovine pericardial tissues for use as a tissue engineering scaffold

机译：牛心包组织脱细胞后的生物力学和结构变化，用作组织工程支架

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摘要

To achieve natural scaffolds for tissue engineering applications we decellularized bovine pericardial (BP) tissues according to two different protocols: a novel treatment based on Triton~® X-100 (12 h, 4℃) (BP1) and a trypsin/ EDTA treatment (37℃,48 h)(BP2). Results were compared with commercially available acellular xenogeneic biomate-rials, Veritas~® and Collamed~®. Biomechanical characteristics, high (E)_h) and low (E_1) modulus of elasticity, of the fresh untreated tissue varied with the anatomical direction (apex to base (T) to transverse (L)) (mean ± SDEV): (41.63 ± 14.65-48.12 ± 10.19 MPa and 0.27 ± 0.05-0.30 ± 0.12 MPa respectively). BP1 had no mechanical effect (44.65 ± 19.73-52.67 ± 7.59 MPa and 0.37 ± 0.14-0.37 ± 0.11 MPa, respectively) but BP2 resulted in significant decrease in E_h and E_1(20.96 ± 8.17-36.82 ± 3.23 MPaand0.20 ± 0.06-0.23 ± 0.06 MPa). Hysteresis ratio (h) varied (19-26 % of the loading energy) independently of anatomical direction. Glycosaminoglycans content was unaffected by BP1, while 22 % of chondroitin/dermatan sulphate and 60 % of hyalu-ronan were removed after BP2 treatment. Endothelial cell adhesion was achieved after 24 h and 3 days cell culture.
机译：为了获得用于组织工程应用的天然支架，我们根据两种不同的方案对牛心包（BP）组织进行了脱细胞处理：一种基于Triton〜®X-100的新型治疗（12 h，4℃）（BP1）和胰蛋白酶/ EDTA处理（ 37℃，48 h）（BP2）。将结果与可商购的脱细胞异种生物材料Veritas®和Collamed®进行比较。未经处理的新鲜组织的生物力学特性（高（E）_h）和低（E_1）弹性模量随解剖学方向（顶点到基础（T）到横向（L））变化（平均值±SDEV）：（41.63± 14.65-48.12±10.19 MPa和0.27±0.05-0.30±0.12 MPa）。 BP1无机械作用（分别为44.65±19.73-52.67±7.59 MPa和0.37±0.14-0.37±0.11 MPa），但BP2导致E_h和E_1显着降低（20.96±8.17-36.82±3.23 MPa和0.20±0.06- 0.23±0.06 MPa）。磁滞比（h）随解剖方向的变化而变化（占加载能量的19-26％）。糖胺聚糖的含量不受BP1的影响，而BP2处理后去除了22％的软骨素/硫酸皮肤素和60％的透明质酸。细胞培养24小时和3天后达到内皮细胞粘附。

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  • 来源
    《Journal of materials science》 |2012年第6期|p.1387-1396|共10页
  • 作者

    Eirini Pagoulatou; Irene-Eva Triantaphyllidou; Demitrios H. Vynios; Dionysios J. Papachristou; Efstratios Koletsis; Despina Deligianni; Dimosthenis Mavrilas;

  • 作者单位

    Laboratory of Biomechanics & Biomedical Engineering,Department of Mechanical Engineering & Aer/tics, University of Patras, 26500 Patras, Greece;

    Laboratory of Biochemistry, Department of Chemistry,University of Patras, Rion, 26500 Patras, Greece;

    Laboratory of Biochemistry, Department of Chemistry,University of Patras, Rion, 26500 Patras, Greece;

    Department of Anatomy-Histology-Embryology, University of Patras, Rion, 26500 Patras, Greece,Department of Pathology, University of Pittsburgh, Pittsburgh,PA, USA;

    Department of Cardiothoracic Surgery, University of Patras,Rion, 26500 Patras, Greece;

    Laboratory of Biomechanics & Biomedical Engineering,Department of Mechanical Engineering & Aer/tics, University of Patras, 26500 Patras, Greece;

    Laboratory of Biomechanics & Biomedical Engineering,Department of Mechanical Engineering & Aer/tics, University of Patras, 26500 Patras, Greece;

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