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首页> 外文期刊>Journal of materials science >Novel pHEMA-gelatin SPHs as bone scaffolds in dynamic cultures
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Novel pHEMA-gelatin SPHs as bone scaffolds in dynamic cultures

机译：新型pHEMA-明胶SPH在动态培养中作为骨支架

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摘要

The effectiveness of poly(2-hydroxyethyl methacrylate)-gelatin superporous hydrogels (pHEMA-gelatin SPHs) was investigated for bone tissue engineering. The cell culture studies were performed with preosteoblas-tic MC3T3-E1 cells. Dynamic culture conditions were provided using 100 ml spinner flask rotating at 50 rpm. According to the results of mitochondrial activity test (1-3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium bromide), there is no significant difference between proliferation behavior of cells cultured under static and dynamic conditions during 28 days. Observations by scanning electron microscopy and confocal laser scanning microscopy showed that, cells attached well onto the scaffolds and spread through the pores for both culture conditions. However, it was found that, calcium deposition and alkaline phosphatase activity in the scaffolds cultured under dynamic conditions were higher than that of static conditions. The expression of osteogenic differentiation markers, i.e. collagen I and osteopontin, based on real-time reverse trans-criptase-polymerase chain reaction demonstrated increased responses under the spinner flask culture conditions. The combination of dynamic culture conditions in spinner flask with the use of superporous pHEMA-gelatin scaffolds enhanced the outcomes related to bone tissue engineering.
机译：研究了聚（甲基丙烯酸2-羟乙酯）-明胶超多孔水凝胶（pHEMA-明胶SPHs）在骨组织工程中的有效性。细胞培养研究使用骨前MC3T3-E1细胞进行。使用以50 rpm旋转的100 ml旋转瓶提供动态培养条件。根据线粒体活性测试结果（1-3- [4,5-二甲基噻唑-2-基]-二苯基四唑溴化物），在静态和动态条件下培养的细胞在28天的增殖行为之间没有显着差异。通过扫描电子显微镜和共聚焦激光扫描显微镜的观察表明，在两种培养条件下，细胞都很好地附着在支架上并通过孔扩散。然而，发现在动态条件下培养的支架中的钙沉积和碱性磷酸酶活性高于静态条件。基于实时逆转录酶-聚合酶链反应的成骨分化标记即胶原I和骨桥蛋白的表达证明在旋转瓶培养条件下反应增强。旋转瓶中动态培养条件与超孔pHEMA-明胶支架的结合使用可增强与骨组织工程相关的结果。

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  • 来源
    《Journal of materials science》 |2012年第11期|2803-2812|共10页
  • 作者

    Damla Cetin; A. Sera Kahraman; Menemse Guemuesderelioglu;

  • 作者单位

    Chemical Engineering Department, Hacettepe University, Beytepe, 06800 Ankara. Turkey;

    Chemical Engineering Department, Hacettepe University, Beytepe, 06800 Ankara. Turkey;

    Chemical Engineering Department, Hacettepe University, Beytepe, 06800 Ankara. Turkey,Bioengineering Department, Hacettepe University, Beytepe, 06800 Ankara, Turkey;

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