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首页> 外文期刊>Journal of Materials Science. Materials in Medicine >The use of long-chain plant polyprenols as a means to modify the biological properties of new biodegradable polyurethane scaffolds for tissue engineering. A pilot study
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The use of long-chain plant polyprenols as a means to modify the biological properties of new biodegradable polyurethane scaffolds for tissue engineering. A pilot study

机译：使用长链植物聚异戊二烯作为一种手段来改变用于组织工程的新型可生物降解聚氨酯支架的生物学特性。初步研究

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Microporous membranes for tissue engineering were produced from new biodegradable polyurethane based on hexamethylene diisocyanate, poly(ε-caprolac-tone) diol and l,4:3,6-dianhydro-D-sorbitol. The interconnected pores had an average size in the range of 5-100 μm. The tensile strength at break, the Young's modulus and elongation at break of the membranes were 3.2 ± 0.3 MPa, 25.2 ± 1.5 MPa and 190 ± 12%, respectively, while non-porous foils from the same polymers had a tensile strength at break of 40 ± 2 MPa, a Young's modulus of 91 ± 6 MPa, and an elongation at break of 370 ± 10%. The membranes were incubated for 10 days in a 2.65 vol% solution of long-chain plant polyprenol in n-hexane to promote their interaction with cells and tissues. The polyprenol was isolated from leaves of Magnolia cobus and was a mixture of prenol-10 and prenol-11. The prenol-impregnated membranes and nonimpregnated membranes (control) were tested in cell culture to assess whether impregnation has a beneficial effect on cell-material interaction. The cells used in the test were chondrocytes isolated from the articular-epiphyseal cartilage of leg bones of 5-day-old inbred LEW rats. The time of culture was 2 and 5 weeks. Both, the nonimpregnated and impregnated polyurethane membranes supported attachment and growth of rat chondrocytes. The cells firmly attached to the surface of the microporous membranes, invaded the pores and maintained the round shape characteristic for chondrocyte-like-morphology. Abundant fibrillar extracellular matrix produced by the cells resembled the network formed by chondrocytes in vivo. The cells produced relatively more extracellular matrix in the membranes impregnated with polyprenol than in the control membranes. Impregnation of polyurethane scaffolds with biologically active amphiphilic polyprenols may be a route to facilitate the cell-material interaction.
机译：用于组织工程的微孔膜是由基于六亚甲基二异氰酸酯，聚（ε-己内酯基）二醇和1,4：3,6-二脱水-D-山梨糖醇的新型可生物降解聚氨酯制成的。互连的孔的平均尺寸在5-100μm的范围内。膜的断裂抗张强度，杨氏模量和断裂伸长率分别为3.2±0.3 MPa，25.2±1.5 MPa和190±12％，而相同聚合物的无孔箔的断裂抗张强度为。 40±2 MPa，杨氏模量为91±6 MPa，断裂伸长率为370±10％。将膜在2.65％（体积）长链植物聚戊二烯的正己烷溶液中孵育10天，以促进其与细胞和组织的相互作用。从木兰的叶中分离出聚戊二烯醇，其为prenol-10和prenol-11的混合物。在细胞培养物中测试了预浸渍酚的膜和未浸渍的膜（对照），以评估浸渍是否对细胞-材料相互作用具有有益的作用。测试中使用的细胞是从5天大的近交LEW大鼠腿骨的关节－pi软骨分离出的软骨细胞。培养时间为2和5周。未浸渍和浸渍的聚氨酯膜均支持大鼠软骨细胞的附着和生长。细胞牢固地附着在微孔膜的表面，侵入毛孔并保持软骨细胞样形态的圆形特征。细胞产生的丰富的纤维状细胞外基质类似于体内软骨细胞形成的网络。与在对照膜中相比，细胞在用聚戊二烯浸渍的膜中产生相对更多的细胞外基质。用具有生物活性的两亲性聚戊二烯浸渍聚氨酯支架可能是促进细胞与材料相互作用的一种途径。

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