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首页> 外文期刊>Journal of materials science >M-keratin nano-materials create a mineralized micro-circumstance to promote proliferation and differentiation of DPSCs
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M-keratin nano-materials create a mineralized micro-circumstance to promote proliferation and differentiation of DPSCs

机译：M-角蛋白纳米材料在矿化微观情况下，促进DPSC的增殖和分化

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摘要

As traditional root canal obturation leads to the loss of the biological activity of the tooth, it is necessary to develop a material that promotes the regeneration of dental tissue. However, this remains a challenging task. Our study aims to construct a mineralized material to support the proliferation and differentiation of dental pulp stem cells (DPSCs), and to explore a new strategy for the treatment of pulp tissue necrosis. Mineralized keratin (M-keratin), defined as keratin that has been mineralized in simulated body fluid, was first harvested to construct the root canal filling material. Characterizations indicated that new substances or components were formed on the surface of keratin particles after mineralization, and the morphology of the keratin was changed. M-keratin promoted the growth, proliferation, and differentiation of DPSCs. After cultivation with M-keratin, DPSCs exhibited more extracellular matrix proteins interacting with the culture interface, the number of these cells increased significantly, and the 3-[4,5-dimethylthiazol-2-yl-]-2,5-diphenyltetrazolium bromide values of cells in the experimental group also increased. Meanwhile, signs that the DPSCs began to differentiate into odontoblasts were observed or detected by alizarin red S staining, ELISA, RNA-Seq, and western blot. We hope that this study will contribute to the development of a new material that promotes the regeneration of dental tissue as well as providing new ideas and strategies for the treatment of dental pulp disease.
机译：随着传统的根管闭合导致牙齿的生物活性丧失，有必要开发促进牙科组织再生的材料。但是，这仍然是一个具有挑战性的任务。我们的研究旨在构建矿化材料以支持牙髓干细胞（DPSC）的增殖和分化，并探讨治疗纸巾坏死的新策略。首先收获定义为已经在模拟体液中被矿化的角蛋白的矿化角蛋白（M-Keratin），以构建根管填充材料。表征表明，在矿化后在角蛋白颗粒表面上形成了新的物质或组分，并且改变了角蛋白的形态。 M-Keratin促进了DPSCS的生长，增殖和分化。用M-keratin培养后，DPSCS表现出与培养界面相互作用的更多细胞外基质蛋白质，这些细胞的数量显着增加，3- [4,5-二甲基噻唑-2-基 - ] - 2,5-二苯基四唑溴铵实验组中细胞的值也增加。同时，探索DPSCS开始将氧化卵细胞分化或检测到胰岛氧化物红S染色，ELISA，RNA-SEQ和Western印迹。我们希望这项研究能够促进促进牙科组织再生的新材料的发展，并为治疗牙髓疾病提供新的思路和策略。

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  • 来源
    《Journal of materials science》 |2020年第12期|124.1-124.13|共13页
  • 作者

    Chen Wu-Ya; Li Xia; Feng Yingying; Lin Siqi; Peng Liwang; Huang Dahong;

  • 作者单位

    Foshan Univ Sch Stomatol & Med Foshan Stomatol Hosp Foshan 528225 Peoples R China|Guangdong Engn Res Ctr Digital Stomatol Foshan 528225 Peoples R China|Foshan Engn Res Ctr Stomatol Foshan 528225 Peoples R China;

    Foshan Univ Sch Stomatol & Med Foshan Stomatol Hosp Foshan 528225 Peoples R China|Guangdong Engn Res Ctr Digital Stomatol Foshan 528225 Peoples R China|Foshan Engn Res Ctr Stomatol Foshan 528225 Peoples R China;

    Foshan Univ Sch Stomatol & Med Foshan Stomatol Hosp Foshan 528225 Peoples R China|Guangdong Engn Res Ctr Digital Stomatol Foshan 528225 Peoples R China|Foshan Engn Res Ctr Stomatol Foshan 528225 Peoples R China;

    Foshan Univ Sch Stomatol & Med Foshan Stomatol Hosp Foshan 528225 Peoples R China|Guangdong Engn Res Ctr Digital Stomatol Foshan 528225 Peoples R China|Foshan Engn Res Ctr Stomatol Foshan 528225 Peoples R China;

    Foshan Univ Sch Stomatol & Med Foshan Stomatol Hosp Foshan 528225 Peoples R China|Guangdong Engn Res Ctr Digital Stomatol Foshan 528225 Peoples R China|Foshan Engn Res Ctr Stomatol Foshan 528225 Peoples R China;

    Foshan Univ Sch Stomatol & Med Foshan Stomatol Hosp Foshan 528225 Peoples R China|Guangdong Engn Res Ctr Digital Stomatol Foshan 528225 Peoples R China|Foshan Engn Res Ctr Stomatol Foshan 528225 Peoples R China;

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