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首页> 外文期刊>Journal of Molecular Histology >Isolation of a native osteoblast matrix with a specific affinity for BMP2
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Isolation of a native osteoblast matrix with a specific affinity for BMP2

机译：对BMP2具有特定亲和力的天然成骨细胞基质的分离

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摘要

During their commitment and differentiation toward the osteoblast lineage, mesenchymal stem cells secrete a unique extracellular matrix (ECM) that contains large quantities of glycosaminoglycans (GAGs). Proteoglycans (PGs) are major structural and functional components of the ECM and are composed of a core protein to which one or more glycosaminoglycan sugar chains (GAGs) attach. The association of BMP2, a member of the TGF-β super-family of growth factors, and a known heparin-binding protein, with GAGs has been implicated as playing a significant role in modulating the growth factor’s in vitro bioactivity. Here we have characterised an osteoblast-derived matrix (MX) obtained from decellularised MC3T3-E1 cell monolayers for its structural attributes, using SEM and histology, and for its functional ability to maintain cell growth and viability. Using a combination of histology and anion exchange chromatography, we first confirmed the retention of GAGs within MX following the decellularisation process. Then the binding specificity of the retained GAG species within the MX for BMP2 was examined using a BMP2-HBP/EGFP (BMP2 Heparin-Binding Peptide/Enhanced Green Fluorescent Protein) fusion protein. The results of this study provide further evidence for a central role of the ECM in the regulation of BMP2 bioactivity, hence on mesenchymal stem cell commitment to the osteoblast lineage.
机译：间充质干细胞在向成骨细胞谱系的定向和分化过程中，会分泌一种独特的细胞外基质（ECM），其中含有大量的糖胺聚糖（GAG）。蛋白聚糖（PG）是ECM的主要结构和功能组件，由一个或多个糖胺聚糖糖链（GAG）连接的核心蛋白组成。 BMP2是生长因子TGF-β超家族的成员，并且是已知的肝素结合蛋白与GAG的关联，被认为在调节生长因子的体外生物活性中起着重要作用。在这里，我们已经表征了从脱细胞的MC3T3-E1细胞单层获得的成骨细胞衍生基质（MX）的结构特征，使用SEM和组织学以及其维持细胞生长和生存能力的功能。使用组织学和阴离子交换色谱法的结合，我们首先确认了脱细胞过程后MX中GAG的保留。然后使用BMP2-HBP / EGFP（BMP2肝素结合肽/绿色荧光蛋白）融合蛋白检查MX中保留的GAG物质对BMP2的结合特异性。这项研究的结果为ECM在调节BMP2生物活性，从而在间充质干细胞对成骨细胞谱系的定位中发挥了重要作用提供了进一步的证据。

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  • 来源
    《Journal of Molecular Histology》 |2007年第5期|393-404|共12页
  • 作者

    Martin Grünert; Christian Dombrowski; Murali Sadasivam; Kerry Manton; Simon M. Cool; Victor Nurcombe;

  • 作者单位

    Stem Cells and Tissue Repair Group Institute of Molecular and Cell Biology Proteos 61 Biopolis Drive Singapore 138673 Singapore;

    Stem Cells and Tissue Repair Group Institute of Molecular and Cell Biology Proteos 61 Biopolis Drive Singapore 138673 Singapore;

    Stem Cells and Tissue Repair Group Institute of Molecular and Cell Biology Proteos 61 Biopolis Drive Singapore 138673 Singapore;

    Stem Cells and Tissue Repair Group Institute of Molecular and Cell Biology Proteos 61 Biopolis Drive Singapore 138673 Singapore;

    Stem Cells and Tissue Repair Group Institute of Molecular and Cell Biology Proteos 61 Biopolis Drive Singapore 138673 Singapore;

    Stem Cells and Tissue Repair Group Institute of Molecular and Cell Biology Proteos 61 Biopolis Drive Singapore 138673 Singapore;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Carbohydrate; Stem cell; Substrate; Growth factor; BMP;

    机译：碳水化合物;干细胞;底物;生长因子;BMP;

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