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首页> 外文期刊>Journal of Nanjing Medical University >Alteration of the Cyclin D1/p16-pRB Pathway, Cellular Proliferation and Apoptosis in Glioma
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Alteration of the Cyclin D1/p16-pRB Pathway, Cellular Proliferation and Apoptosis in Glioma

机译：胶质瘤细胞周期蛋白D1 / p16-pRB途径的改变，细胞增殖和凋亡

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Objective: To study the alteration of cyclin D1, p16 and pKB in glioma, analyze proliferation and ap-optosis of tumor cells, and discuss the pathogenesis of glioma. Methods: Thirty-seven glioma specimens were classified as astrocytoma(25 cases, including 7 fibrillary cases; 6 protoplasmic cases; 12 anaplastic cases), and glioblastoma(12 cases, including 4 GBM cases). Ten normal brain tissues were taken as controls. The expression of cyclin D1, p16 and pRB were detected by immunohistochemical method. Cellular proliferation was assessed by Ki-67 label index(Ki-67 LI) .Cellular apoptosis was detected by TDNEL and apoptotic indices(AI)was calculated. Results: The alterations of three proteins were cyclin D1 overexpression(28/37,75.7%), p16 and pKB deletion(20/37,54.1 % and 12/37, 32.4%) which were closely related to tumor types, particularly in malignant glioma. Ki-67 LI and AI were higher when pRB pathway was abnormal . Apoptosis was minor in astrocytic tumors (astrocytomas, 0.010 +- 0.002; glioblastomas, 0.057 +- 0.016). Conclusion: The abnormalities of cyclin D1/p16-pRB pathway correlated closely with pathogenesis of glioma.
机译：目的：研究神经胶质瘤中cyclin D1，p16和pKB的变化，分析肿瘤细胞的增殖和凋亡，探讨神经胶质瘤的发病机制。方法：将37例神经胶质瘤标本分为星形细胞瘤（25例，其中7例为原纤维瘤； 6例原浆性； 12例间变性）和胶质母细胞瘤（12例，其中4例GBM）。将十个正常脑组织作为对照。免疫组化法检测cyclin D1，p16和pRB的表达。用Ki-67标记指数（Ki-67 LI）评估细胞增殖，用TDNEL法检测细胞凋亡，并计算凋亡指数（AI）。结果：三种蛋白的变化为细胞周期蛋白D1过表达（28 / 37,75.7％），p16和pKB缺失（20 / 37,54.1％和12/37，32.4％），这与肿瘤类型密切相关，尤其是恶性肿瘤。胶质瘤。 pRB途径异常时，Ki-67 LI和AI较高。在星形细胞肿瘤中凋亡较小（星形细胞瘤，0.010±0.002；成胶质细胞瘤，0.057±0.016）。结论：细胞周期蛋白D1 / p16-pRB通路异常与神经胶质瘤的发病机制密切相关。

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